{
 "exported_at": "2026-10-28T23:55:17.444567+00:00",
 "exported_by": {
  "app": "quantetta-a web",
  "code": {
   "git_commit": "",
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 "recording_short": "mouse_2609_150ms_control_iDS",
 "header": {
  "short": "mouse_2609_150ms_control_iDS",
  "label": "Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_iDS",
  "facts": "200 \u00d7 200 px, 1,954 frames, 0.977 kHz, pixel size not stated, deposit checks: negative values",
  "run_short": "ee804172",
  "run_when": "2026-10-28 20:47 UTC",
  "params_version": 3,
  "commit": "0d2391b",
  "dirty": false,
  "workers": 4,
  "sig_short": "4091a5ca",
  "run_status": "done",
  "protocol_name": "Mouse whole heart optical mapping (Luther lab)"
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 "parameters": "% Mouse whole heart optical mapping (Luther lab), v3, read-only\n% set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16\nbaseline_frames  = 901;     % no baseline rule recorded for this run\npad_frames       = 500;     % each side, outside the window\nspatial_sigma    = 1.0;     % px, Gaussian, phase only\nspatial_truncate = 4.0;     % kernel radius, sigma\nlowpass          = [];      % none\npolarity         = 1;       % measured, skew of dV/dt\nrotor_threshold  = 0.9;     % turns, |winding| per plaquette\nrotor_erode_px   = 3;       % px from tissue edge ignored\nlink_px          = 4.0;     % tracking, max step per frame\nmax_gap          = 5;       % frames a track may vanish\nmin_cycles       = 1.0;     % own rotations, min_frames = 136\npair_dist_px     = 3.0;     % opposite-charge pairing\npaired_frac      = 0.5;     % max paired fraction of a track\nrender_step      = 4;       % movie only",
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   "start": 0,
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   "cycle": "[not measured]",
   "expected": "not stated",
   "bounded": "recording start \u2192 recording end",
   "defined_by": "admission (project lead); no comparison defined",
   "citation": null,
   "provisional": true
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      "persistent_rotor_frames_frac": 0.7861635220125787
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 },
 "critic": {
  "code": {
   "dirty": false,
   "git_commit": "0d2391b04113d316716bce2e2f9ee6ec6728d5d3"
  },
  "agent": "critic",
  "model": "openai:gpt-5.5",
  "checks": {
   "checks": {
    "persistent_rotor_tracks": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotor_tracks",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": null,
     "spread_frac": 2.19,
     "value_at_run_setting": 308,
     "range_across_settings": [
      9.0,
      609.0
     ]
    },
    "whole_recording.dominant_freq_hz": {
     "unit": "Hz",
     "range": null,
     "metric": "dominant_freq_hz",
     "stable": true,
     "status": "no_cited_range",
     "segment": "whole_recording",
     "spread_frac": 0.154,
     "value_at_run_setting": 7.169,
     "range_across_settings": [
      6.145,
      7.169
     ]
    },
    "whole_recording.persistent_rotors": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotors",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": "whole_recording",
     "spread_frac": 2.19,
     "value_at_run_setting": 308,
     "range_across_settings": [
      9.0,
      609.0
     ]
    },
    "whole_recording.singularity_frames_frac": {
     "unit": "fraction of frames",
     "range": null,
     "metric": "singularity_frames_frac",
     "stable": true,
     "status": "no_cited_range",
     "segment": "whole_recording",
     "spread_frac": 0.056,
     "value_at_run_setting": 1.0,
     "range_across_settings": [
      0.9444444444444444,
      1.0
     ]
    },
    "whole_recording.persistent_rotor_count_max": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotor_count_max",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": "whole_recording",
     "spread_frac": 3.524,
     "value_at_run_setting": 26,
     "range_across_settings": [
      1.0,
      75.0
     ]
    }
   },
   "unstable": [
    "whole_recording.persistent_rotors",
    "whole_recording.persistent_rotor_count_max",
    "persistent_rotor_tracks"
   ],
   "physiology_ok": null,
   "ranged_checks": []
  },
  "ranges": [
   {
    "id": "mouse_spiral_rotation_hz",
    "low": 18.75,
    "high": 20.0,
    "unit": "Hz",
    "scope": "one optical mapping recording in the same study (spiral wave before and during illumination); not a population range",
    "metric": "dominant_freq_hz",
    "source": {
     "ref": "10.1371/journal.pcbi.1011660",
     "quotes": [
      "rotational frequency of \u2248 20 Hz before illumination",
      "the frequency of the arrhythmia decreases to \u2248 18\\.75 Hz"
     ],
     "passages": [
      " is shown in Fig 3B. It shows that the arrhythmia consists of a single spiral wave with a stationary core dynamic and a rotational frequency of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia de",
      "ncy of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia decreases to \u2248 18.75 Hz. It is important to note that the frequency reverses its original value of \u2248 20 Hz when the light is turned off. Fig 3C"
     ]
    },
    "status": "provisional",
    "tissue": "whole_heart",
    "species": "mouse",
    "applies_to": {
     "segment_expected_persistent_rotors": "present"
    },
    "checked_low": 14.0625,
    "checked_high": 25.0,
    "tolerance_frac": 0.25
   }
  ],
  "run_id": "ee804172-2876-47fb-94df-d522ea5c6e03",
  "status": "provisional",
  "verdict": {
   "send_back": false,
   "critic_notes": "Verdict: no cited-range physiology pass/fail can be assigned, and the ranges, the stability threshold of 0.25 x median, and the criteria are provisional; reportable descriptive outputs are singularity_frames_frac with range 0.9444444444444444 to 1.0 fraction of frames and dominant_freq_hz with range 6.145 to 7.169 Hz, while whole_recording.persistent_rotors and persistent_rotor_tracks are unstable at 9.0 to 609.0 and whole_recording.persistent_rotor_count_max is unstable at 1.0 to 75.0, so those counts are not reportable as single values; review the undocumented all-negative deposited values, low-confidence polarity, whole-frame mask, provisional SNR/processing thresholds, and tracker sensitivity of 80, 308, and 64 rotors, with mouse spiral-wave context from DOI 10.1371/journal.pcbi.1011660.",
   "review_items": [
    {
     "topic": "unstable:whole_recording.persistent_rotors",
     "detail": "The metric varies from 9.0 to 609.0 across filter grid and tracker settings and is not reportable as a single count.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "unstable:whole_recording.persistent_rotor_count_max",
     "detail": "The metric varies from 1.0 to 75.0 across filter grid and tracker settings and is not reportable as a single maximum count.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "unstable:persistent_rotor_tracks",
     "detail": "The metric varies from 9.0 to 609.0 across filter grid and tracker settings and is not reportable as a single track count.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "Undocumented pre-deposit transformation",
     "detail": "The deposited file appears transformed before deposit based on all-negative integer values, and the deposit metadata and paper text do not explain that transformation.",
     "severity": "warn",
     "reviewer_type": "data_inspector"
    },
    {
     "topic": "Polarity confidence",
     "detail": "Measured polarity is not inverted, but confidence is low; use pipeline measurement-based orientation rather than assuming raw Di-4-ANEPPS fluorescence polarity.",
     "severity": "warn",
     "reviewer_type": "data_inspector"
    },
    {
     "topic": "Whole-frame mask",
     "detail": "No depositor mask exists and the generated mask may include background as tissue; replace or check the mask before spatial interpretation.",
     "severity": "warn",
     "reviewer_type": "data_inspector"
    },
    {
     "topic": "SNR and processing thresholds are provisional",
     "detail": "SNR and the flags used to infer prior processing come from provisional tool thresholds and methods, and temporal smoothing may inflate SNR.",
     "severity": "info",
     "reviewer_type": "data_inspector"
    },
    {
     "topic": "tracker count sensitivity",
     "detail": "Strict, default, and loose tracker settings returned 80, 308, and 64 persistent rotors, while the rule verdict agrees; this should be reviewed as sensitivity rather than taken as proof the qualitative finding is false.",
     "severity": "warn",
     "reviewer_type": "analysis"
    }
   ],
   "tracker_note": "Strict, default, and loose trackers returned 80, 308, and 64 persistent rotors for whole_recording. The tracker comparison reports agree=true for the rule verdict, but the count sensitivity should be reviewed rather than treated as falsifying the finding.",
   "physiology_ok": null,
   "metric_judgments": [
    {
     "note": "Stable across settings, with range 0.9444444444444444 to 1.0 fraction of frames, but there is no cited physiology range for this metric, so it may be reported only as a descriptive analysis output rather than a pass/fail physiology claim.",
     "metric": "whole_recording.singularity_frames_frac",
     "status": "no_cited_range"
    },
    {
     "note": "Unstable across filter grid and tracker settings, ranging from 9.0 to 609.0, so the persistent-rotor count is not reportable as a single signature value. Mouse spiral-wave context in DOI 10.1371/journal.pcbi.1011660 describes a single spiral wave with \u2248 20 Hz rotational frequency before illumination, but this tool found no cited range for this count metric.",
     "metric": "whole_recording.persistent_rotors",
     "status": "unstable_not_reportable"
    },
    {
     "note": "Unstable across filter grid and tracker settings, ranging from 1.0 to 75.0, so the maximum simultaneous persistent-rotor count is not reportable as a single signature value.",
     "metric": "whole_recording.persistent_rotor_count_max",
     "status": "unstable_not_reportable"
    },
    {
     "note": "Stable across baseline windows, with range 6.145 to 7.169 Hz, but no cited range was applied to this recording, so it may be reported only descriptively and not interpreted as within or outside a validated physiology range.",
     "metric": "whole_recording.dominant_freq_hz",
     "status": "no_cited_range"
    },
    {
     "note": "Unstable across filter grid and tracker settings, ranging from 9.0 to 609.0 tracks, so the track count is not reportable as a single signature value.",
     "metric": "persistent_rotor_tracks",
     "status": "unstable_not_reportable"
    }
   ],
   "send_back_reason": null,
   "persistence_threshold_note": "Not applicable: the measured-cycle rule was not applied because there were no segment expectations, and the paper-rotation rule was also not applicable because there were no segment expectations; agreement is null."
  },
  "passages": {
   "mouse_spiral_rotation_period": {
    "ref": "10.1371/journal.pcbi.1011660",
    "note": "At 1000 frames/s, 20 Hz is a 50-frame rotation; the illuminated value of 18.75 Hz would be 53 frames.",
    "quotes": [
     "rotational frequency of \u2248 20 Hz before illumination"
    ],
    "status": "provisional",
    "passages": [
     " is shown in Fig 3B. It shows that the arrhythmia consists of a single spiral wave with a stationary core dynamic and a rotational frequency of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia de"
    ],
    "applies_to": {
     "species": "mouse"
    },
    "rotation_hz": 20.0
   }
  },
  "trackers": {
   "note": "disagreement is reported as a review item, not folded into the pass/fail",
   "agree": true,
   "trackers": [
    {
     "link_px": 2.0,
     "max_gap": 1,
     "tracker": "strict",
     "is_run_setting": false,
     "persistent_rotors_by_segment": {
      "whole_recording": 80
     }
    },
    {
     "link_px": 4.0,
     "max_gap": 5,
     "tracker": "default",
     "is_run_setting": true,
     "persistent_rotors_by_segment": {
      "whole_recording": 308
     }
    },
    {
     "link_px": 6.0,
     "max_gap": 10,
     "tracker": "loose",
     "is_run_setting": false,
     "persistent_rotors_by_segment": {
      "whole_recording": 64
     }
    }
   ]
  },
  "stability": {
   "persistent_rotor_tracks": {
    "max": 609.0,
    "min": 9.0,
    "median": 274.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 2.19,
    "values_grid": [
     12,
     274,
     329,
     11,
     351,
     609,
     16,
     229,
     305,
     9,
     308,
     370
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     80,
     308,
     64
    ]
   },
   "whole_recording.dominant_freq_hz": {
    "max": 7.169,
    "min": 6.145,
    "median": 6.657,
    "stable": true,
    "settings": 4,
    "spread_frac": 0.154,
    "varied_over": "baseline windows",
    "values_by_baseline": {
     "204": 6.145,
     "408": 7.169,
     "816": 6.145,
     "901": 7.169
    }
   },
   "whole_recording.persistent_rotors": {
    "max": 609.0,
    "min": 9.0,
    "median": 274.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 2.19,
    "values_grid": [
     12,
     274,
     329,
     11,
     351,
     609,
     16,
     229,
     305,
     9,
     308,
     370
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     80,
     308,
     64
    ]
   },
   "whole_recording.singularity_frames_frac": {
    "max": 1.0,
    "min": 0.9444444444444444,
    "median": 1.0,
    "stable": true,
    "settings": 12,
    "spread_frac": 0.056,
    "values_grid": [
     1.0,
     1.0,
     0.9486373165618449,
     1.0,
     1.0,
     0.9863731656184487,
     1.0,
     1.0,
     0.9706498951781971,
     1.0,
     1.0,
     0.9444444444444444
    ],
    "varied_over": "filter grid"
   },
   "whole_recording.persistent_rotor_present": {
    "stable": true,
    "values_grid": [
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     true,
     true,
     true
    ],
    "stable_across_filters": true,
    "stable_across_trackers": true
   },
   "whole_recording.persistent_rotor_count_max": {
    "max": 75.0,
    "min": 1.0,
    "median": 21.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 3.524,
    "values_grid": [
     2,
     24,
     37,
     2,
     21,
     75,
     2,
     17,
     23,
     1,
     26,
     54
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     12,
     26,
     10
    ]
   }
  },
  "reviewed_at": "2026-10-28T20:51:25.681458+00:00",
  "recording_id": "669d42ed-e0bf-4229-9a5f-9301454eafe0",
  "signature_id": "4091a5ca-4825-4b42-bced-67d6ecc7a015",
  "prompt_version": "critic-v1",
  "criteria_status": {
   "note": null,
   "rules": [
    {
     "id": "physiology_reference_rates",
     "expert": "cardiac electrophysiologist per species",
     "status": "provisional",
     "values": {
      "ranges": [
       "mouse_spiral_rotation_hz"
      ],
      "tolerance_frac": 0.25,
      "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
     },
     "decision": null,
     "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
     "reviewed": false,
     "reviewer": null,
     "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
     "reviewed_at": null
    },
    {
     "id": "stability_rule",
     "expert": "biostatistician + optical mapping analyst",
     "status": "provisional",
     "values": {
      "unstable": [
       "whole_recording.persistent_rotors",
       "whole_recording.persistent_rotor_count_max",
       "persistent_rotor_tracks"
      ],
      "stable_frac": 0.25
     },
     "decision": null,
     "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
     "reviewed": false,
     "reviewer": null,
     "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
     "reviewed_at": null
    },
    {
     "id": "persistence_threshold_variants",
     "expert": "computational cardiac electrophysiologist (phase mapping)",
     "status": "provisional",
     "values": {
      "measured_cycle": 136,
      "paper_rotation": null
     },
     "decision": null,
     "location": "worker/agents/critic.py (persistence_variants)",
     "reviewed": false,
     "reviewer": null,
     "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
     "reviewed_at": null
    },
    {
     "id": "persistent_rotor_criterion",
     "expert": "cardiac electrophysiologist",
     "status": "provisional",
     "values": {
      "max_absent": 0,
      "min_present": 1
     },
     "decision": null,
     "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
     "reviewed": false,
     "reviewer": null,
     "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
     "reviewed_at": null
    },
    {
     "id": "sensitivity_grid",
     "expert": "optical mapping analyst",
     "status": "provisional",
     "values": {
      "spatial_sigma": {
       "light": 0.5,
       "default": 1.0,
       "heavier": 2.0
      },
      "baseline_frames": {
       "run": 901,
       "3x_cycle": 408,
       "6x_cycle": 816,
       "1.5x_cycle": 204
      },
      "baseline_scaling": {
       "multiples": {
        "3x_cycle": 3.0,
        "6x_cycle": 6.0,
        "1.5x_cycle": 1.5
       },
       "reference_segment": "whole_recording",
       "segment_cycles_frames": {
        "whole_recording": 136
       },
       "reference_cycle_frames": 136,
       "window_over_cycle_per_segment": {
        "run": {
         "whole_recording": 6.62
        },
        "3x_cycle": {
         "whole_recording": 3.0
        },
        "6x_cycle": {
         "whole_recording": 6.0
        },
        "1.5x_cycle": {
         "whole_recording": 1.5
        }
       }
      },
      "tracker(link_px, max_gap)": {
       "loose": [
        6.0,
        10
       ],
       "strict": [
        2.0,
        1
       ],
       "default": [
        4.0,
        5
       ]
      }
     },
     "decision": null,
     "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
     "reviewed": false,
     "reviewer": null,
     "description": "Filter levels tested: spatial sigma and baseline window values",
     "reviewed_at": null
    }
   ],
   "status": "provisional",
   "all_reviewed": false,
   "superseded_by_run": null
  },
  "persistence_variants": {
   "agree": null,
   "measured_cycle": {
    "basis": "one dominant cycle of the fastest segment at the run setting",
    "reason": "no segment expectations, so the rule has nothing to test",
    "applicable": false,
    "min_frames": 136
   },
   "paper_rotation": {
    "reason": "no segment expectations",
    "applicable": false
   }
  },
  "required_review_topics": [
   "unstable:whole_recording.persistent_rotors",
   "unstable:whole_recording.persistent_rotor_count_max",
   "unstable:persistent_rotor_tracks"
  ]
 },
 "criteria": [
  {
   "criterion": "Design expectations",
   "rule": "none stated for this recording's segments",
   "result": "no criterion to test; counts are descriptive",
   "provisional": true
  }
 ],
 "report": {
  "methods": "Using provisional parameter set version 3 for protocol f65d786f-f194-4be2-88b8-10d3dd25d674, the provisional fixed criteria were applied with baseline_frames=901, spatial_sigma=1.0, spatial_truncate=4.0, pad_frames=500, rotor_threshold=0.9, rotor_erode_px=3, link_px=4.0, max_gap=5, min_cycles=1.0, pair_dist_px=3.0, paired_frac=0.5, and render_step=4. The recording has 1954 frames of 200 x 200 pixels in mat format; the frame rate is 977.0 Hz from the recording-row admission citing the paper methods quote that recordings were 200x200 pixels at 977 frames per second, and the pixel size is not available. The analysed frames are 500 to 1454, and the provisional segment definition is one whole_recording segment, labelled whole recording, from start_frame=0 to end_frame=1954, defined at admission with no detected or logged events.",
  "rules": [
   {
    "id": "protocol_parameter_set",
    "description": "Each protocol's fixed, versioned analysis parameters (version 1 = developer defaults); no run or agent may deviate",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/protocol_params.py (developer_defaults, set_params)",
    "values": {
     "set_by": "developer (not a domain scientist)",
     "protocol": "Mouse whole heart optical mapping (Luther lab)",
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
     "params_version": 3
    }
   },
   {
    "id": "baseline_window_rule",
    "description": "A run proceeds only if the protocol's baseline window is >= 2x the slowest segment's cycle, the cycle from the MAP channel when a DAQ log exists, else from per-pixel dominant frequency on linearly detrended pixels; otherwise the run is held by code",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/baseline_rule.py (FACTOR, evaluate)",
    "values": {
     "ok": null,
     "factor": 2.0,
     "source": "per-pixel dominant frequency (images)",
     "required_min_frames": null,
     "slowest_cycle_frames": null
    }
   },
   {
    "id": "filter_defaults",
    "description": "Default baseline window (frames) and spatial Gaussian sigma/truncate for phase",
    "expert": "optical mapping analyst (cardiac electrophysiology)",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (Params)",
    "values": {
     "lowpass": null,
     "spatial_sigma": 1.0,
     "baseline_frames": 901,
     "spatial_truncate": 4.0
    }
   },
   {
    "id": "tissue_mask",
    "description": "Tissue mask from, in order: (1) NaN in frame 0 when the recording has any NaN; (2) a mask or background-image variable shipped in the file whose shape matches the frame (binary as is, an intensity image thresholded with Otsu); (3) Otsu's threshold on the mean image, the class with more temporal signal as tissue, largest connected component kept; source, coverage and threshold recorded",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/io.py (tissue_mask_info, otsu_threshold, largest_component)",
    "values": {
     "rule": "intensity variable 'bgimage' in the file, Otsu threshold 1628, class above with more temporal signal, largest connected component",
     "side": "above",
     "source": "file_variable",
     "coverage": 0.44555,
     "variable": "bgimage",
     "threshold": 1627.92578125,
     "tissue_pixels": 17822
    }
   },
   {
    "id": "polarity",
    "description": "Dye polarity from sign of median per-pixel skew of dV/dt",
    "expert": "optical mapping analyst / voltage dye chemist",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (polarity)",
    "values": {
     "method": "median skew of dV/dt",
     "result": 1
    }
   },
   {
    "id": "singularity_detection",
    "description": "Phase singularity = |winding| > threshold turns, >= erode px from tissue edge",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (Params.rotor_threshold, rotor_erode_px)",
    "values": {
     "rotor_erode_px": 3,
     "rotor_threshold": 0.9
    }
   },
   {
    "id": "rotor_persistence",
    "description": "Track linking distance/gap and minimum lifetime for a persistent rotor",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/rotors.py (TrackParams)",
    "values": {
     "link_px": 4.0,
     "max_gap": 5,
     "min_cycles": 1.0,
     "min_frames": 136
    }
   },
   {
    "id": "chirality_pairing",
    "description": "Opposite-charge singularities within pair distance are a pair; tracks paired most of their life are excluded from rotors",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/rotors.py (TrackParams.pair_dist_px, paired_frac)",
    "values": {
     "paired_frac": 0.5,
     "pair_dist_px": 3.0
    }
   },
   {
    "id": "sensitivity_grid",
    "description": "Filter levels tested: spatial sigma and baseline window values",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
    "values": {
     "sigmas": {
      "light": 0.5,
      "default": 1.0,
      "heavier": 2.0
     },
     "trackers": {
      "loose": [
       6.0,
       10
      ],
      "strict": [
       2.0,
       1
      ],
      "default": [
       4.0,
       5
      ]
     },
     "baselines": {
      "run": 901,
      "3x_cycle": 408,
      "6x_cycle": 816,
      "1.5x_cycle": 204
     }
    }
   },
   {
    "id": "analysis_window",
    "description": "Frames analysed: whole recording minus one filter pad at each end (unless the plan narrows it)",
    "expert": "cardiac electrophysiologist familiar with the dataset",
    "status": "provisional",
    "location": "worker/voltage/runs.py (default_window)",
    "values": {
     "f0": 500,
     "f1": 1454
    }
   },
   {
    "id": "prior_processing_flags",
    "description": "Thresholds for flagging depositor processing (normalization, masking, temporal/spatial smoothing)",
    "expert": "imaging scientist (camera acquisition and noise)",
    "status": "provisional",
    "location": "worker/voltage/inspect.py (prior_processing)",
    "values": {
     "flags": [
      "negative_values"
     ],
     "thresholds": {
      "int_frac": 0.5,
      "neg_frac": 0.01,
      "dv_autocorr": 0.5,
      "neighbour_corr": 0.8
     }
    }
   },
   {
    "id": "pipeline_check",
    "description": "The pipeline agent's hold/proceed decision from protocol mismatches and inspector anomalies; it chooses no parameter",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/agents/pipeline.py (INSTRUCTIONS, validator)",
    "values": {
     "model": "openai:gpt-5.5",
     "proceed": true,
     "anomalies": [
      "Undocumented pre-deposit transformation",
      "Polarity confidence",
      "Whole-frame mask",
      "baseline-window rule"
     ],
     "mismatches": []
    }
   },
   {
    "id": "physiology_reference_rates",
    "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
    "expert": "cardiac electrophysiologist per species",
    "status": "provisional",
    "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
    "values": {
     "ranges": [
      "mouse_spiral_rotation_hz"
     ],
     "tolerance_frac": 0.25,
     "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
    }
   },
   {
    "id": "stability_rule",
    "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
    "expert": "biostatistician + optical mapping analyst",
    "status": "provisional",
    "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
    "values": {
     "unstable": [
      "whole_recording.persistent_rotors",
      "whole_recording.persistent_rotor_count_max",
      "persistent_rotor_tracks"
     ],
     "stable_frac": 0.25
    }
   },
   {
    "id": "persistence_threshold_variants",
    "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/agents/critic.py (persistence_variants)",
    "values": {
     "measured_cycle": 136,
     "paper_rotation": null
    }
   },
   {
    "id": "persistent_rotor_criterion",
    "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
    "expert": "cardiac electrophysiologist",
    "status": "provisional",
    "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
    "values": {
     "max_absent": 0,
     "min_present": 1
    }
   }
  ],
  "review_items": [
   {
    "topic": "unstable:whole_recording.persistent_rotors",
    "detail": "The metric varies from 9.0 to 609.0 across filter grid and tracker settings and is not reportable as a single count.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "unstable:whole_recording.persistent_rotor_count_max",
    "detail": "The metric varies from 1.0 to 75.0 across filter grid and tracker settings and is not reportable as a single maximum count.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "unstable:persistent_rotor_tracks",
    "detail": "The metric varies from 9.0 to 609.0 across filter grid and tracker settings and is not reportable as a single track count.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "Undocumented pre-deposit transformation",
    "detail": "The deposited file appears transformed before deposit based on all-negative integer values, and the deposit metadata and paper text do not explain that transformation.",
    "severity": "warn",
    "reviewer": "data_inspector"
   },
   {
    "topic": "Polarity confidence",
    "detail": "Measured polarity is not inverted, but confidence is low; use pipeline measurement-based orientation rather than assuming raw Di-4-ANEPPS fluorescence polarity.",
    "severity": "warn",
    "reviewer": "data_inspector"
   },
   {
    "topic": "Whole-frame mask",
    "detail": "No depositor mask exists and the generated mask may include background as tissue; replace or check the mask before spatial interpretation.",
    "severity": "warn",
    "reviewer": "data_inspector"
   },
   {
    "topic": "SNR and processing thresholds are provisional",
    "detail": "SNR and the flags used to infer prior processing come from provisional tool thresholds and methods, and temporal smoothing may inflate SNR.",
    "severity": "info",
    "reviewer": "data_inspector"
   },
   {
    "topic": "tracker count sensitivity",
    "detail": "Strict, default, and loose tracker settings returned 80, 308, and 64 persistent rotors, while the rule verdict agrees; this should be reviewed as sensitivity rather than taken as proof the qualitative finding is false.",
    "severity": "warn",
    "reviewer": "analysis"
   }
  ],
  "reportable": [
   {
    "metric": "persistent rotor tracks",
    "segment": "all",
    "value": "9\u2013609",
    "unit": "count",
    "status": "unstable, not reportable"
   },
   {
    "metric": "dominant freq (Hz)",
    "segment": "whole recording",
    "value": "7.169",
    "unit": "Hz",
    "status": "no cited range"
   },
   {
    "metric": "persistent rotors",
    "segment": "whole recording",
    "value": "9\u2013609",
    "unit": "count",
    "status": "unstable, not reportable"
   },
   {
    "metric": "singularity frames fraction",
    "segment": "whole recording",
    "value": "1",
    "unit": "fraction of frames",
    "status": "no cited range"
   },
   {
    "metric": "persistent rotor count max",
    "segment": "whole recording",
    "value": "1\u201375",
    "unit": "count",
    "status": "unstable, not reportable"
   }
  ],
  "verdict": {
   "physiology_ok": null,
   "send_back": false,
   "notes": "Verdict: no cited-range physiology pass/fail can be assigned, and the ranges, the stability threshold of 0.25 x median, and the criteria are provisional; reportable descriptive outputs are singularity_frames_frac with range 0.9444444444444444 to 1.0 fraction of frames and dominant_freq_hz with range 6.145 to 7.169 Hz, while whole_recording.persistent_rotors and persistent_rotor_tracks are unstable at 9.0 to 609.0 and whole_recording.persistent_rotor_count_max is unstable at 1.0 to 75.0, so those counts are not reportable as single values; review the undocumented all-negative deposited values, low-confidence polarity, whole-frame mask, provisional SNR/processing thresholds, and tracker sensitivity of 80, 308, and 64 rotors, with mouse spiral-wave context from DOI 10.1371/journal.pcbi.1011660."
  },
  "provenance": {
   "recording": {
    "id": "669d42ed-e0bf-4229-9a5f-9301454eafe0",
    "label": "Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_iDS",
    "source": "admission",
    "source_ref": "10.5281/zenodo.5557829",
    "publication_ref": "10.1371/journal.pone.0259174",
    "storage_path": "uploads/public/7764050e/mouse_2609_150ms_control_iDS.mat",
    "format": "mat",
    "sha256": "f9f4309fb395aa43ccae96f6e85724a7a739a98c295ff45598a6417b01605709",
    "size_bytes": 114024466,
    "license": "cc-by-4.0",
    "attribution": "Rybashlykov Dmitry",
    "rights_source": {
     "ref": "10.5281/zenodo.5557829",
     "kind": "deposit_metadata",
     "admitted_by": "project lead",
     "license_as_stated": "cc-by-4.0"
    },
    "frame_rate_hz": 977.0,
    "frame_rate_source": {
     "by": "project lead",
     "ref": "10.1371/journal.pone.0259174",
     "kind": "admission",
     "quote": "The custom software was designed to make recordings with a resolution of 200x200 pixels and a framerate of 977 frames per second (FPS).",
     "source": "paper_methods"
    },
    "pixel_size_um": null,
    "pixel_size_source": null,
    "frames": 1954,
    "height": 200,
    "width": 200,
    "inverted": false
   },
   "protocol": {
    "id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
    "name": "Mouse whole heart optical mapping (Luther lab)",
    "species": "mouse",
    "tissue": "whole_heart",
    "params_version": 3,
    "params_set_by": "developer (not a domain scientist)",
    "params_note": "version 3: baseline 901 frames = 2.1 x the 433-frame (2.31 Hz) post-termination cycle resolved under v2; v2's 641 frames was 1.48 cycles, on the 1.5-cycle line, and the pipeline check held one run and passed another on the same inputs. pad 500 >= half the window. Provisional (REVIEW_NEEDED item 16)."
   },
   "run": {
    "id": "ee804172-2876-47fb-94df-d522ea5c6e03",
    "status": "done",
    "started_at": "2026-10-28T20:47:10.483007+00:00",
    "finished_at": "2026-10-28T20:51:26.621979+00:00",
    "code": {
     "dirty": false,
     "git_commit": "0d2391b04113d316716bce2e2f9ee6ec6728d5d3"
    },
    "workers": 4,
    "steps": [
     "load",
     "deposit_checks",
     "baseline_correct",
     "polarity",
     "spatial_filter(phase only)",
     "hilbert_phase",
     "phase_singularities",
     "track_and_pair",
     "beats_apd_cv",
     "sensitivity",
     "render"
    ],
    "protocol_params": {
     "params": {
      "link_px": 4.0,
      "max_gap": 5,
      "min_cycles": 1.0,
      "pad_frames": 500,
      "paired_frac": 0.5,
      "render_step": 4,
      "pair_dist_px": 3.0,
      "spatial_sigma": 1.0,
      "rotor_erode_px": 3,
      "baseline_frames": 901,
      "rotor_threshold": 0.9,
      "spatial_truncate": 4.0
     },
     "version": 3,
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674"
    },
    "baseline_rule": {
     "ok": null,
     "cycles": {
      "whole_recording": {
       "n_frames": 1954,
       "cycle_frames": null,
       "pixels_with_rhythm_frac": 0.0
      }
     },
     "factor": 2.0,
     "reason": "no segment cycle could be measured",
     "source": "per-pixel dominant frequency (images)",
     "status": "provisional",
     "baseline_frames": 901
    },
    "deposit_checks": {
     "flags": [
      {
       "flag": "negative_values",
       "value": 1.0,
       "suggests": "baseline-subtracted or normalized; raw fluorescence counts are non-negative",
       "threshold": 0.01
      }
     ],
     "status": "provisional",
     "measures": {
      "max": -4.0,
      "min": -7378.0,
      "frames_used": 1954,
      "integer_frac": 1.0,
      "negative_frac": 1.0,
      "frames_with_nan": 0,
      "dv_lag1_autocorr_median": -0.3829070739238835,
      "neighbour_dv_corr_median": 0.23243111156884547
     },
     "processed_before_deposit": true
    },
    "pipeline_check": {
     "model": "openai:gpt-5.5",
     "proceed": true,
     "anomalies": [
      {
       "topic": "Undocumented pre-deposit transformation",
       "severity": "warn",
       "effect_on_analysis": "The fixed pipeline can run, but the deposited values are all negative integers rather than raw non-negative fluorescence. Polarity, baseline correction, amplitudes and any interpretation of preprocessing-dependent quantities should be read as applying to this transformed deposit, not to raw camera fluorescence; prior filtering or sign/offset changes may make the total preprocessing unknown."
      },
      {
       "topic": "Polarity confidence",
       "severity": "warn",
       "effect_on_analysis": "The analysis should use the pipeline's measurement-based orientation rather than dye expectation. Because the measured skew is weak and block agreement is incomplete, activation/upstroke orientation is less certain, although this is not by itself a parameter mismatch."
      },
      {
       "topic": "Whole-frame mask",
       "severity": "warn",
       "effect_on_analysis": "The available mask covers the full 200 x 200 frame because no depositor mask or NaN mask is present. Spatial summaries and phase/rotor analyses may include background unless the generated tissue mask is checked or replaced outside this fixed-parameter run."
      },
      {
       "topic": "baseline-window rule",
       "severity": "info",
       "effect_on_analysis": "The coded baseline-window rule returned ok=null with reason 'no segment cycle could be measured' from per-pixel dominant frequency images, using factor 2.0 and baseline_frames 901. Per instruction this is reported only as information and does not hold the run."
      }
     ],
     "rationale": "The recording matches the protocol at the level of the available facts: it is a mouse whole-heart optical mapping deposit from the referenced study, and there is no conflicting protocol-specified frame rate, indicator, pixel size or image size. The fixed version 3 parameters are therefore applicable as the provisional protocol settings for this recording, with important caveats: the deposited data appear transformed before deposit, the polarity measurement is low-confidence, and the automatic mask is the whole frame. These issues affect interpretation and quality control of the fixed pipeline outputs but do not create a blocking mismatch in the protocol parameters.",
     "mismatches": [],
     "hold_reason": null,
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
     "methods_text": "Using provisional parameter set version 3 for protocol f65d786f-f194-4be2-88b8-10d3dd25d674, the provisional fixed criteria were applied with baseline_frames=901, spatial_sigma=1.0, spatial_truncate=4.0, pad_frames=500, rotor_threshold=0.9, rotor_erode_px=3, link_px=4.0, max_gap=5, min_cycles=1.0, pair_dist_px=3.0, paired_frac=0.5, and render_step=4. The recording has 1954 frames of 200 x 200 pixels in mat format; the frame rate is 977.0 Hz from the recording-row admission citing the paper methods quote that recordings were 200x200 pixels at 977 frames per second, and the pixel size is not available. The analysed frames are 500 to 1454, and the provisional segment definition is one whole_recording segment, labelled whole recording, from start_frame=0 to end_frame=1954, defined at admission with no detected or logged events.",
     "recording_id": "669d42ed-e0bf-4229-9a5f-9301454eafe0",
     "params_version": 3,
     "prompt_version": "pipeline-v2"
    }
   },
   "signature": {
    "id": "4091a5ca-4825-4b42-bced-67d6ecc7a015",
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       "location": "worker/voltage/rotors.py (TrackParams.pair_dist_px, paired_frac)",
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        ],
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   "label": "Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_iDS",
   "source": "admission",
   "source_ref": "10.5281/zenodo.5557829",
   "publication_ref": "10.1371/journal.pone.0259174",
   "storage_path": "uploads/public/7764050e/mouse_2609_150ms_control_iDS.mat",
   "format": "mat",
   "sha256": "f9f4309fb395aa43ccae96f6e85724a7a739a98c295ff45598a6417b01605709",
   "size_bytes": 114024466,
   "license": "cc-by-4.0",
   "attribution": "Rybashlykov Dmitry",
   "rights_source": {
    "ref": "10.5281/zenodo.5557829",
    "kind": "deposit_metadata",
    "admitted_by": "project lead",
    "license_as_stated": "cc-by-4.0"
   },
   "frame_rate_hz": 977.0,
   "frame_rate_source": {
    "by": "project lead",
    "ref": "10.1371/journal.pone.0259174",
    "kind": "admission",
    "quote": "The custom software was designed to make recordings with a resolution of 200x200 pixels and a framerate of 977 frames per second (FPS).",
    "source": "paper_methods"
   },
   "pixel_size_um": null,
   "pixel_size_source": null,
   "frames": 1954,
   "height": 200,
   "width": 200,
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  },
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   "id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
   "name": "Mouse whole heart optical mapping (Luther lab)",
   "species": "mouse",
   "tissue": "whole_heart",
   "params_version": 3,
   "params_set_by": "developer (not a domain scientist)",
   "params_note": "version 3: baseline 901 frames = 2.1 x the 433-frame (2.31 Hz) post-termination cycle resolved under v2; v2's 641 frames was 1.48 cycles, on the 1.5-cycle line, and the pipeline check held one run and passed another on the same inputs. pad 500 >= half the window. Provisional (REVIEW_NEEDED item 16)."
  },
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   "id": "ee804172-2876-47fb-94df-d522ea5c6e03",
   "status": "done",
   "started_at": "2026-10-28T20:47:10.483007+00:00",
   "finished_at": "2026-10-28T20:51:26.621979+00:00",
   "code": {
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    "git_commit": "0d2391b04113d316716bce2e2f9ee6ec6728d5d3"
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   "workers": 4,
   "steps": [
    "load",
    "deposit_checks",
    "baseline_correct",
    "polarity",
    "spatial_filter(phase only)",
    "hilbert_phase",
    "phase_singularities",
    "track_and_pair",
    "beats_apd_cv",
    "sensitivity",
    "render"
   ],
   "protocol_params": {
    "params": {
     "link_px": 4.0,
     "max_gap": 5,
     "min_cycles": 1.0,
     "pad_frames": 500,
     "paired_frac": 0.5,
     "render_step": 4,
     "pair_dist_px": 3.0,
     "spatial_sigma": 1.0,
     "rotor_erode_px": 3,
     "baseline_frames": 901,
     "rotor_threshold": 0.9,
     "spatial_truncate": 4.0
    },
    "version": 3,
    "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674"
   },
   "baseline_rule": {
    "ok": null,
    "cycles": {
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      "n_frames": 1954,
      "cycle_frames": null,
      "pixels_with_rhythm_frac": 0.0
     }
    },
    "factor": 2.0,
    "reason": "no segment cycle could be measured",
    "source": "per-pixel dominant frequency (images)",
    "status": "provisional",
    "baseline_frames": 901
   },
   "deposit_checks": {
    "flags": [
     {
      "flag": "negative_values",
      "value": 1.0,
      "suggests": "baseline-subtracted or normalized; raw fluorescence counts are non-negative",
      "threshold": 0.01
     }
    ],
    "status": "provisional",
    "measures": {
     "max": -4.0,
     "min": -7378.0,
     "frames_used": 1954,
     "integer_frac": 1.0,
     "negative_frac": 1.0,
     "frames_with_nan": 0,
     "dv_lag1_autocorr_median": -0.3829070739238835,
     "neighbour_dv_corr_median": 0.23243111156884547
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    "processed_before_deposit": true
   },
   "pipeline_check": {
    "model": "openai:gpt-5.5",
    "proceed": true,
    "anomalies": [
     {
      "topic": "Undocumented pre-deposit transformation",
      "severity": "warn",
      "effect_on_analysis": "The fixed pipeline can run, but the deposited values are all negative integers rather than raw non-negative fluorescence. Polarity, baseline correction, amplitudes and any interpretation of preprocessing-dependent quantities should be read as applying to this transformed deposit, not to raw camera fluorescence; prior filtering or sign/offset changes may make the total preprocessing unknown."
     },
     {
      "topic": "Polarity confidence",
      "severity": "warn",
      "effect_on_analysis": "The analysis should use the pipeline's measurement-based orientation rather than dye expectation. Because the measured skew is weak and block agreement is incomplete, activation/upstroke orientation is less certain, although this is not by itself a parameter mismatch."
     },
     {
      "topic": "Whole-frame mask",
      "severity": "warn",
      "effect_on_analysis": "The available mask covers the full 200 x 200 frame because no depositor mask or NaN mask is present. Spatial summaries and phase/rotor analyses may include background unless the generated tissue mask is checked or replaced outside this fixed-parameter run."
     },
     {
      "topic": "baseline-window rule",
      "severity": "info",
      "effect_on_analysis": "The coded baseline-window rule returned ok=null with reason 'no segment cycle could be measured' from per-pixel dominant frequency images, using factor 2.0 and baseline_frames 901. Per instruction this is reported only as information and does not hold the run."
     }
    ],
    "rationale": "The recording matches the protocol at the level of the available facts: it is a mouse whole-heart optical mapping deposit from the referenced study, and there is no conflicting protocol-specified frame rate, indicator, pixel size or image size. The fixed version 3 parameters are therefore applicable as the provisional protocol settings for this recording, with important caveats: the deposited data appear transformed before deposit, the polarity measurement is low-confidence, and the automatic mask is the whole frame. These issues affect interpretation and quality control of the fixed pipeline outputs but do not create a blocking mismatch in the protocol parameters.",
    "mismatches": [],
    "hold_reason": null,
    "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
    "methods_text": "Using provisional parameter set version 3 for protocol f65d786f-f194-4be2-88b8-10d3dd25d674, the provisional fixed criteria were applied with baseline_frames=901, spatial_sigma=1.0, spatial_truncate=4.0, pad_frames=500, rotor_threshold=0.9, rotor_erode_px=3, link_px=4.0, max_gap=5, min_cycles=1.0, pair_dist_px=3.0, paired_frac=0.5, and render_step=4. The recording has 1954 frames of 200 x 200 pixels in mat format; the frame rate is 977.0 Hz from the recording-row admission citing the paper methods quote that recordings were 200x200 pixels at 977 frames per second, and the pixel size is not available. The analysed frames are 500 to 1454, and the provisional segment definition is one whole_recording segment, labelled whole recording, from start_frame=0 to end_frame=1954, defined at admission with no detected or logged events.",
    "recording_id": "669d42ed-e0bf-4229-9a5f-9301454eafe0",
    "params_version": 3,
    "prompt_version": "pipeline-v2"
   }
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   "created_at": "2026-10-28T20:50:40.714483+00:00",
   "movie_path": "runs/ee804172-2876-47fb-94df-d522ea5c6e03/movie.mp4",
   "frames": {
    "f0": 500,
    "f1": 1454,
    "maps": "runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/maps.npz",
    "dtype": "<f4",
    "phase": "runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/phase.npy",
    "shape": [
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     200,
     200
    ],
    "sha256": {
     "maps": "febd1fedf4c5ac367f926ee31d180e1aab1d706d2a508aae05ecad79a2aec05a",
     "phase": "2b59c98a1241c41c4686211bbd340f252a47a248feee3fb1d1bf7483064de237",
     "voltage": "e9da51c3d0d7dbdfd641c20930ab62c145eae47daeb674c3b3455bb85ec4b7f1"
    },
    "voltage": "runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/voltage.npy",
    "map_keys": [
     "dominant_frequency:whole_recording",
     "dominant_frequency:window",
     "mask"
    ],
    "map_units": {
     "mask": "tissue mask, source file_variable",
     "dominant_frequency:window": "Hz",
     "dominant_frequency:whole_recording": "Hz"
    }
   },
   "filter_params": {
    "mask": "not NaN in frame 0 and nonzero temporal std",
    "render": {
     "step": 4,
     "palettes": {
      "phase": "twilight",
      "camera": "gray",
      "voltage": "gray"
     },
     "interpolation": "nearest"
    },
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 }
}