{
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   "git_commit": "",
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  "short": "mouse_2609_150ms_control_MiCAM",
  "label": "Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_MiCAM",
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  "run_when": "2026-10-28 18:15 UTC",
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       {
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       {
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       {
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       {
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       {
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       {
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       },
       {
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       }
      ],
      "persistent_rotor_frames_frac": 1.0
     }
    },
    "is_run_setting": false,
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   }
  ]
 },
 "critic": {
  "code": {
   "dirty": true,
   "git_commit": "008c2dc076bcebf9e7fa01a05e8b7c4b5a713b37"
  },
  "agent": "critic",
  "model": "openai:gpt-5.5",
  "checks": {
   "checks": {
    "persistent_rotor_tracks": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotor_tracks",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": null,
     "spread_frac": 4.66,
     "value_at_run_setting": 1118,
     "range_across_settings": [
      1.0,
      5211.0
     ]
    },
    "whole_recording.dominant_freq_hz": {
     "unit": "Hz",
     "range": null,
     "metric": "dominant_freq_hz",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": "whole_recording",
     "spread_frac": 0.579,
     "value_at_run_setting": 55.989,
     "range_across_settings": [
      55.989,
      107.311
     ]
    },
    "whole_recording.persistent_rotors": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotors",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": "whole_recording",
     "spread_frac": 4.66,
     "value_at_run_setting": 1118,
     "range_across_settings": [
      1.0,
      5211.0
     ]
    },
    "whole_recording.singularity_frames_frac": {
     "unit": "fraction of frames",
     "range": null,
     "metric": "singularity_frames_frac",
     "stable": true,
     "status": "no_cited_range",
     "segment": "whole_recording",
     "spread_frac": 0.0,
     "value_at_run_setting": 1.0,
     "range_across_settings": [
      1.0,
      1.0
     ]
    },
    "whole_recording.persistent_rotor_count_max": {
     "unit": "count",
     "range": null,
     "metric": "persistent_rotor_count_max",
     "stable": false,
     "status": "unstable_not_reportable",
     "segment": "whole_recording",
     "spread_frac": 2.86,
     "value_at_run_setting": 43,
     "range_across_settings": [
      1.0,
      124.0
     ]
    }
   },
   "unstable": [
    "whole_recording.persistent_rotors",
    "whole_recording.persistent_rotor_count_max",
    "whole_recording.dominant_freq_hz",
    "persistent_rotor_tracks"
   ],
   "physiology_ok": null,
   "ranged_checks": []
  },
  "ranges": [
   {
    "id": "mouse_spiral_rotation_hz",
    "low": 18.75,
    "high": 20.0,
    "unit": "Hz",
    "scope": "one optical mapping recording in the same study (spiral wave before and during illumination); not a population range",
    "metric": "dominant_freq_hz",
    "source": {
     "ref": "10.1371/journal.pcbi.1011660",
     "quotes": [
      "rotational frequency of \u2248 20 Hz before illumination",
      "the frequency of the arrhythmia decreases to \u2248 18\\.75 Hz"
     ],
     "passages": [
      " is shown in Fig 3B. It shows that the arrhythmia consists of a single spiral wave with a stationary core dynamic and a rotational frequency of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia de",
      "ncy of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia decreases to \u2248 18.75 Hz. It is important to note that the frequency reverses its original value of \u2248 20 Hz when the light is turned off. Fig 3C"
     ]
    },
    "status": "provisional",
    "tissue": "whole_heart",
    "species": "mouse",
    "applies_to": {
     "segment_expected_persistent_rotors": "present"
    },
    "checked_low": 14.0625,
    "checked_high": 25.0,
    "tolerance_frac": 0.25
   }
  ],
  "run_id": "6e21b7f8-7cd1-499c-8e8f-ab37b36c7113",
  "status": "provisional",
  "verdict": {
   "send_back": false,
   "critic_notes": "Verdict: no physiology pass/fail can be computed because no stable ranged checks are available; the provisional ranges, stability threshold, and criteria should be treated as provisional. What is reportable is the stable whole-recording singularity-frames fraction, range 1.0 to 1.0, only as an observed output without a cited physiological range. What is not reportable as single values is persistent rotors, range 1.0 to 5211.0; maximum concurrent persistent-rotor count, range 1.0 to 124.0; dominant frequency, range 55.989 to 107.311 Hz; and persistent rotor tracks, range 1.0 to 5211.0. A same-species mouse spiral-wave context passage is available, but dominant-frequency comparison should wait because this metric is unstable (DOI 10.1371/journal.pcbi.1011660). Review the tracker count sensitivity, with strict/default/loose counts of 327, 1118, and 94, and the inspector concerns about 977.0 Hz MiCAM frame-rate provenance, likely pre-deposit sign or conditioning, all-negative integer data values, rhythm-detector unsuitability for the expected paced period, and the non-biological mask.",
   "review_items": [
    {
     "topic": "unstable:whole_recording.persistent_rotors",
     "detail": "Persistent-rotor count varies across the range 1.0 to 5211.0 across filter and tracker settings, exceeding the provisional stability rule.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "unstable:whole_recording.persistent_rotor_count_max",
     "detail": "Maximum concurrent persistent-rotor count varies across the range 1.0 to 124.0 across filter and tracker settings, exceeding the provisional stability rule.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "unstable:whole_recording.dominant_freq_hz",
     "detail": "Dominant frequency varies across the range 55.989 to 107.311 Hz across baseline windows, exceeding the provisional stability rule.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "unstable:persistent_rotor_tracks",
     "detail": "Persistent rotor tracks vary across the range 1.0 to 5211.0 across filter and tracker settings, exceeding the provisional stability rule.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "tracker_count_sensitivity",
     "detail": "Tracker variants produced different counts: strict 327, default 1118, and loose 94, so count magnitude needs tracking-parameter review even though rule-level agreement is true.",
     "severity": "warn",
     "reviewer_type": "analysis"
    },
    {
     "topic": "Frame-rate provenance for a MiCAM file",
     "detail": "Use 977.0 Hz as the working frame rate, but the cited paper quote is for the custom system whereas this file is labelled MiCAM; Zenodo metadata does not state the MiCAM frame rate.",
     "severity": "warn",
     "reviewer_type": "inspector"
    },
    {
     "topic": "Pre-deposit sign/conditioning",
     "detail": "Measured polarity is consistently non-inverted despite Di-4-ANEPPS normally dimming on depolarization, suggesting a sign flip or conditioning before deposit or export.",
     "severity": "warn",
     "reviewer_type": "inspector"
    },
    {
     "topic": "Unexplained all-negative data values",
     "detail": "All values are negative integers and are flagged as processed_before_deposit; the linked paper documents possible workflows, but the deposit README does not explain this file-level transformation.",
     "severity": "warn",
     "reviewer_type": "inspector"
    },
    {
     "topic": "Rhythm detector not suitable for the expected paced period here",
     "detail": "The rhythm tool result should not be used for physiology or frame-rate inference here because the expected paced period lies beyond the rhythm tool's baseline-window suitability.",
     "severity": "info",
     "reviewer_type": "inspector"
    },
    {
     "topic": "Mask is not a heart segmentation",
     "detail": "The mask is based only on nonzero temporal standard deviation and covers almost the entire image; tissue-only spatial interpretation needs a biological mask.",
     "severity": "warn",
     "reviewer_type": "inspector"
    }
   ],
   "tracker_note": "Strict, default, and loose tracking found 327, 1118, and 94 persistent rotors in the whole recording, respectively. The rule-level tracker comparison is marked as agreeing, but the large count spread remains a review item and should not be treated as evidence that the rotor count is quantitatively stable.",
   "physiology_ok": null,
   "metric_judgments": [
    {
     "note": "Stable across the filter grid at the range 1.0 to 1.0 fraction of frames, but no cited comparator range is available, so it can be reported only as an observed analysis output, not as a physiology-validated finding.",
     "metric": "whole_recording.singularity_frames_frac",
     "status": "no_cited_range"
    },
    {
     "note": "Unstable across filter and tracker settings, spanning the range 1.0 to 5211.0 counts; do not report a single rotor-count value from this signature without parameter-sensitivity review.",
     "metric": "whole_recording.persistent_rotors",
     "status": "unstable_not_reportable"
    },
    {
     "note": "Unstable across filter and tracker settings, spanning the range 1.0 to 124.0 counts; do not report the maximum concurrent rotor-count value as a single finding.",
     "metric": "whole_recording.persistent_rotor_count_max",
     "status": "unstable_not_reportable"
    },
    {
     "note": "Unstable across baseline windows, spanning the range 55.989 to 107.311 Hz; this should not be used as the reported dominant frequency. A same-species optical-mapping context passage is available for comparison only after the frequency estimate is stable (DOI 10.1371/journal.pcbi.1011660).",
     "metric": "whole_recording.dominant_freq_hz",
     "status": "unstable_not_reportable"
    },
    {
     "note": "Unstable across filter and tracker settings, spanning the range 1.0 to 5211.0 tracks; track counts should be withheld pending review of detection and tracking sensitivity.",
     "metric": "persistent_rotor_tracks",
     "status": "unstable_not_reportable"
    }
   ],
   "send_back_reason": null,
   "persistence_threshold_note": "Persistent-rotor threshold checks are not applicable at either the measured-cycle basis or the paper-rotation-period basis because there are no segment expectations; the agreement flag is not applicable."
  },
  "passages": {
   "mouse_spiral_rotation_period": {
    "ref": "10.1371/journal.pcbi.1011660",
    "note": "At 1000 frames/s, 20 Hz is a 50-frame rotation; the illuminated value of 18.75 Hz would be 53 frames.",
    "quotes": [
     "rotational frequency of \u2248 20 Hz before illumination"
    ],
    "status": "provisional",
    "passages": [
     " is shown in Fig 3B. It shows that the arrhythmia consists of a single spiral wave with a stationary core dynamic and a rotational frequency of \u2248 20 Hz before illumination. During a single global optical pulse with a PL of 5000 ms and an LI of 3.21 \u03bcW/mm2, the frequency of the arrhythmia de"
    ],
    "applies_to": {
     "species": "mouse"
    },
    "rotation_hz": 20.0
   }
  },
  "trackers": {
   "note": "disagreement is reported as a review item, not folded into the pass/fail",
   "agree": true,
   "trackers": [
    {
     "link_px": 2.0,
     "max_gap": 1,
     "tracker": "strict",
     "is_run_setting": false,
     "persistent_rotors_by_segment": {
      "whole_recording": 327
     }
    },
    {
     "link_px": 4.0,
     "max_gap": 5,
     "tracker": "default",
     "is_run_setting": true,
     "persistent_rotors_by_segment": {
      "whole_recording": 1118
     }
    },
    {
     "link_px": 6.0,
     "max_gap": 10,
     "tracker": "loose",
     "is_run_setting": false,
     "persistent_rotors_by_segment": {
      "whole_recording": 94
     }
    }
   ]
  },
  "stability": {
   "persistent_rotor_tracks": {
    "max": 5211.0,
    "min": 1.0,
    "median": 1118.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 4.66,
    "values_grid": [
     5,
     1161,
     4500,
     6,
     1239,
     5175,
     1,
     1297,
     5211,
     7,
     1118,
     3969
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     327,
     1118,
     94
    ]
   },
   "whole_recording.dominant_freq_hz": {
    "max": 107.311,
    "min": 55.989,
    "median": 88.6485,
    "stable": false,
    "settings": 4,
    "spread_frac": 0.579,
    "varied_over": "baseline windows",
    "values_by_baseline": {
     "26": 71.852,
     "51": 107.311,
     "102": 105.445,
     "901": 55.989
    }
   },
   "whole_recording.persistent_rotors": {
    "max": 5211.0,
    "min": 1.0,
    "median": 1118.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 4.66,
    "values_grid": [
     5,
     1161,
     4500,
     6,
     1239,
     5175,
     1,
     1297,
     5211,
     7,
     1118,
     3969
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     327,
     1118,
     94
    ]
   },
   "whole_recording.singularity_frames_frac": {
    "max": 1.0,
    "min": 1.0,
    "median": 1.0,
    "stable": true,
    "settings": 12,
    "spread_frac": 0.0,
    "values_grid": [
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0,
     1.0
    ],
    "varied_over": "filter grid"
   },
   "whole_recording.persistent_rotor_present": {
    "stable": true,
    "values_grid": [
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true,
     true
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     true,
     true,
     true
    ],
    "stable_across_filters": true,
    "stable_across_trackers": true
   },
   "whole_recording.persistent_rotor_count_max": {
    "max": 124.0,
    "min": 1.0,
    "median": 43.0,
    "stable": false,
    "settings": 15,
    "spread_frac": 2.86,
    "values_grid": [
     3,
     48,
     114,
     2,
     49,
     119,
     1,
     43,
     124,
     2,
     43,
     118
    ],
    "varied_over": "filter grid + trackers",
    "values_trackers": [
     10,
     43,
     12
    ]
   }
  },
  "reviewed_at": "2026-10-28T18:18:30.467102+00:00",
  "recording_id": "ae77081e-a125-471f-8805-27dad0688848",
  "signature_id": "804ddd67-f7b9-45c6-a823-bef795065584",
  "prompt_version": "critic-v1",
  "criteria_status": {
   "note": null,
   "rules": [
    {
     "id": "physiology_reference_rates",
     "expert": "cardiac electrophysiologist per species",
     "status": "provisional",
     "values": {
      "ranges": [
       "mouse_spiral_rotation_hz"
      ],
      "tolerance_frac": 0.25,
      "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
     },
     "decision": null,
     "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
     "reviewed": false,
     "reviewer": null,
     "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
     "reviewed_at": null
    },
    {
     "id": "stability_rule",
     "expert": "biostatistician + optical mapping analyst",
     "status": "provisional",
     "values": {
      "unstable": [
       "whole_recording.persistent_rotors",
       "whole_recording.persistent_rotor_count_max",
       "whole_recording.dominant_freq_hz",
       "persistent_rotor_tracks"
      ],
      "stable_frac": 0.25
     },
     "decision": null,
     "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
     "reviewed": false,
     "reviewer": null,
     "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
     "reviewed_at": null
    },
    {
     "id": "persistence_threshold_variants",
     "expert": "computational cardiac electrophysiologist (phase mapping)",
     "status": "provisional",
     "values": {
      "measured_cycle": 17,
      "paper_rotation": null
     },
     "decision": null,
     "location": "worker/agents/critic.py (persistence_variants)",
     "reviewed": false,
     "reviewer": null,
     "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
     "reviewed_at": null
    },
    {
     "id": "persistent_rotor_criterion",
     "expert": "cardiac electrophysiologist",
     "status": "provisional",
     "values": {
      "max_absent": 0,
      "min_present": 1
     },
     "decision": null,
     "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
     "reviewed": false,
     "reviewer": null,
     "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
     "reviewed_at": null
    },
    {
     "id": "sensitivity_grid",
     "expert": "optical mapping analyst",
     "status": "provisional",
     "values": {
      "spatial_sigma": {
       "light": 0.5,
       "default": 1.0,
       "heavier": 2.0
      },
      "baseline_frames": {
       "run": 901,
       "3x_cycle": 51,
       "6x_cycle": 102,
       "1.5x_cycle": 26
      },
      "baseline_scaling": {
       "multiples": {
        "3x_cycle": 3.0,
        "6x_cycle": 6.0,
        "1.5x_cycle": 1.5
       },
       "reference_segment": "whole_recording",
       "segment_cycles_frames": {
        "whole_recording": 17
       },
       "reference_cycle_frames": 17,
       "window_over_cycle_per_segment": {
        "run": {
         "whole_recording": 53.0
        },
        "3x_cycle": {
         "whole_recording": 3.0
        },
        "6x_cycle": {
         "whole_recording": 6.0
        },
        "1.5x_cycle": {
         "whole_recording": 1.53
        }
       }
      },
      "tracker(link_px, max_gap)": {
       "loose": [
        6.0,
        10
       ],
       "strict": [
        2.0,
        1
       ],
       "default": [
        4.0,
        5
       ]
      }
     },
     "decision": null,
     "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
     "reviewed": false,
     "reviewer": null,
     "description": "Filter levels tested: spatial sigma and baseline window values",
     "reviewed_at": null
    }
   ],
   "status": "provisional",
   "all_reviewed": false,
   "superseded_by_run": null
  },
  "persistence_variants": {
   "agree": null,
   "measured_cycle": {
    "basis": "one dominant cycle of the fastest segment at the run setting",
    "reason": "no segment expectations, so the rule has nothing to test",
    "applicable": false,
    "min_frames": 17
   },
   "paper_rotation": {
    "reason": "no segment expectations",
    "applicable": false
   }
  },
  "required_review_topics": [
   "unstable:whole_recording.persistent_rotors",
   "unstable:whole_recording.persistent_rotor_count_max",
   "unstable:whole_recording.dominant_freq_hz",
   "unstable:persistent_rotor_tracks"
  ]
 },
 "criteria": [
  {
   "criterion": "Design expectations",
   "rule": "none stated for this recording's segments",
   "result": "no criterion to test; counts are descriptive",
   "provisional": true
  }
 ],
 "report": {
  "methods": "Using provisional protocol f65d786f-f194-4be2-88b8-10d3dd25d674 with provisional parameter set version 3, the fixed analysis parameters were baseline_frames 901, spatial_sigma 1.0, spatial_truncate 4.0, pad_frames 500, rotor_threshold 0.9, rotor_erode_px 3, link_px 4.0, max_gap 5, min_cycles 1.0, pair_dist_px 3.0, paired_frac 0.5, and render_step 4. The recording has 2047 frames of 100 by 100 pixels in mat format; the frame rate used was 977.0 Hz from the recording-row admission entry citing 10.1371/journal.pone.0259174, and the pixel size was not available. The analysed window used for rhythm reporting was frames 500 to 1547, and the provisional segment definition was one whole_recording segment spanning frames 0 to 2047; the parameter set, segment definitions, and criteria are provisional.",
  "rules": [
   {
    "id": "protocol_parameter_set",
    "description": "Each protocol's fixed, versioned analysis parameters (version 1 = developer defaults); no run or agent may deviate",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/protocol_params.py (developer_defaults, set_params)",
    "values": {
     "set_by": "developer (not a domain scientist)",
     "protocol": "Mouse whole heart optical mapping (Luther lab)",
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
     "params_version": 3
    }
   },
   {
    "id": "baseline_window_rule",
    "description": "A run proceeds only if the protocol's baseline window is >= 2x the slowest segment's cycle, the cycle from the MAP channel when a DAQ log exists, else from per-pixel dominant frequency on linearly detrended pixels; otherwise the run is held by code",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/baseline_rule.py (FACTOR, evaluate)",
    "values": {
     "ok": true,
     "factor": 2.0,
     "source": "per-pixel dominant frequency (images)",
     "required_min_frames": 14,
     "slowest_cycle_frames": 7
    }
   },
   {
    "id": "filter_defaults",
    "description": "Default baseline window (frames) and spatial Gaussian sigma/truncate for phase",
    "expert": "optical mapping analyst (cardiac electrophysiology)",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (Params)",
    "values": {
     "lowpass": null,
     "spatial_sigma": 1.0,
     "baseline_frames": 901,
     "spatial_truncate": 4.0
    }
   },
   {
    "id": "tissue_mask",
    "description": "Tissue = not NaN in frame 0 and nonzero temporal std",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/io.py (tissue_mask)",
    "values": {
     "rule": "not NaN in frame 0 and nonzero std",
     "tissue_pixels": 9999
    }
   },
   {
    "id": "polarity",
    "description": "Dye polarity from sign of median per-pixel skew of dV/dt",
    "expert": "optical mapping analyst / voltage dye chemist",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (polarity)",
    "values": {
     "method": "median skew of dV/dt",
     "result": 1
    }
   },
   {
    "id": "singularity_detection",
    "description": "Phase singularity = |winding| > threshold turns, >= erode px from tissue edge",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/preprocess.py (Params.rotor_threshold, rotor_erode_px)",
    "values": {
     "rotor_erode_px": 3,
     "rotor_threshold": 0.9
    }
   },
   {
    "id": "rotor_persistence",
    "description": "Track linking distance/gap and minimum lifetime for a persistent rotor",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/rotors.py (TrackParams)",
    "values": {
     "link_px": 4.0,
     "max_gap": 5,
     "min_cycles": 1.0,
     "min_frames": 17
    }
   },
   {
    "id": "chirality_pairing",
    "description": "Opposite-charge singularities within pair distance are a pair; tracks paired most of their life are excluded from rotors",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/voltage/rotors.py (TrackParams.pair_dist_px, paired_frac)",
    "values": {
     "paired_frac": 0.5,
     "pair_dist_px": 3.0
    }
   },
   {
    "id": "sensitivity_grid",
    "description": "Filter levels tested: spatial sigma and baseline window values",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
    "values": {
     "sigmas": {
      "light": 0.5,
      "default": 1.0,
      "heavier": 2.0
     },
     "trackers": {
      "loose": [
       6.0,
       10
      ],
      "strict": [
       2.0,
       1
      ],
      "default": [
       4.0,
       5
      ]
     },
     "baselines": {
      "run": 901,
      "3x_cycle": 51,
      "6x_cycle": 102,
      "1.5x_cycle": 26
     }
    }
   },
   {
    "id": "analysis_window",
    "description": "Frames analysed: whole recording minus one filter pad at each end (unless the plan narrows it)",
    "expert": "cardiac electrophysiologist familiar with the dataset",
    "status": "provisional",
    "location": "worker/voltage/runs.py (default_window)",
    "values": {
     "f0": 500,
     "f1": 1547
    }
   },
   {
    "id": "prior_processing_flags",
    "description": "Thresholds for flagging depositor processing (normalization, masking, temporal/spatial smoothing)",
    "expert": "imaging scientist (camera acquisition and noise)",
    "status": "provisional",
    "location": "worker/voltage/inspect.py (prior_processing)",
    "values": {
     "flags": [
      "negative_values"
     ],
     "thresholds": {
      "int_frac": 0.5,
      "neg_frac": 0.01,
      "dv_autocorr": 0.5,
      "neighbour_corr": 0.8
     }
    }
   },
   {
    "id": "pipeline_check",
    "description": "The pipeline agent's hold/proceed decision from protocol mismatches and inspector anomalies; it chooses no parameter",
    "expert": "optical mapping analyst",
    "status": "provisional",
    "location": "worker/agents/pipeline.py (INSTRUCTIONS, validator)",
    "values": {
     "model": "openai:gpt-5.5",
     "proceed": true,
     "anomalies": [
      "Frame-rate provenance for a MiCAM file",
      "Pre-deposit sign/conditioning",
      "Unexplained all-negative data values",
      "Mask is not a heart segmentation",
      "Rhythm detector not suitable for the expected paced period here",
      "baseline-window rule"
     ],
     "mismatches": []
    }
   },
   {
    "id": "physiology_reference_rates",
    "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
    "expert": "cardiac electrophysiologist per species",
    "status": "provisional",
    "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
    "values": {
     "ranges": [
      "mouse_spiral_rotation_hz"
     ],
     "tolerance_frac": 0.25,
     "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
    }
   },
   {
    "id": "stability_rule",
    "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
    "expert": "biostatistician + optical mapping analyst",
    "status": "provisional",
    "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
    "values": {
     "unstable": [
      "whole_recording.persistent_rotors",
      "whole_recording.persistent_rotor_count_max",
      "whole_recording.dominant_freq_hz",
      "persistent_rotor_tracks"
     ],
     "stable_frac": 0.25
    }
   },
   {
    "id": "persistence_threshold_variants",
    "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
    "expert": "computational cardiac electrophysiologist (phase mapping)",
    "status": "provisional",
    "location": "worker/agents/critic.py (persistence_variants)",
    "values": {
     "measured_cycle": 17,
     "paper_rotation": null
    }
   },
   {
    "id": "persistent_rotor_criterion",
    "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
    "expert": "cardiac electrophysiologist",
    "status": "provisional",
    "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
    "values": {
     "max_absent": 0,
     "min_present": 1
    }
   }
  ],
  "review_items": [
   {
    "topic": "unstable:whole_recording.persistent_rotors",
    "detail": "Persistent-rotor count varies across the range 1.0 to 5211.0 across filter and tracker settings, exceeding the provisional stability rule.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "unstable:whole_recording.persistent_rotor_count_max",
    "detail": "Maximum concurrent persistent-rotor count varies across the range 1.0 to 124.0 across filter and tracker settings, exceeding the provisional stability rule.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "unstable:whole_recording.dominant_freq_hz",
    "detail": "Dominant frequency varies across the range 55.989 to 107.311 Hz across baseline windows, exceeding the provisional stability rule.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "unstable:persistent_rotor_tracks",
    "detail": "Persistent rotor tracks vary across the range 1.0 to 5211.0 across filter and tracker settings, exceeding the provisional stability rule.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "tracker_count_sensitivity",
    "detail": "Tracker variants produced different counts: strict 327, default 1118, and loose 94, so count magnitude needs tracking-parameter review even though rule-level agreement is true.",
    "severity": "warn",
    "reviewer": "analysis"
   },
   {
    "topic": "Frame-rate provenance for a MiCAM file",
    "detail": "Use 977.0 Hz as the working frame rate, but the cited paper quote is for the custom system whereas this file is labelled MiCAM; Zenodo metadata does not state the MiCAM frame rate.",
    "severity": "warn",
    "reviewer": "inspector"
   },
   {
    "topic": "Pre-deposit sign/conditioning",
    "detail": "Measured polarity is consistently non-inverted despite Di-4-ANEPPS normally dimming on depolarization, suggesting a sign flip or conditioning before deposit or export.",
    "severity": "warn",
    "reviewer": "inspector"
   },
   {
    "topic": "Unexplained all-negative data values",
    "detail": "All values are negative integers and are flagged as processed_before_deposit; the linked paper documents possible workflows, but the deposit README does not explain this file-level transformation.",
    "severity": "warn",
    "reviewer": "inspector"
   },
   {
    "topic": "Rhythm detector not suitable for the expected paced period here",
    "detail": "The rhythm tool result should not be used for physiology or frame-rate inference here because the expected paced period lies beyond the rhythm tool's baseline-window suitability.",
    "severity": "info",
    "reviewer": "inspector"
   },
   {
    "topic": "Mask is not a heart segmentation",
    "detail": "The mask is based only on nonzero temporal standard deviation and covers almost the entire image; tissue-only spatial interpretation needs a biological mask.",
    "severity": "warn",
    "reviewer": "inspector"
   }
  ],
  "reportable": [
   {
    "metric": "persistent rotor tracks",
    "segment": "all",
    "value": "1\u20135.21e+03",
    "unit": "count",
    "status": "unstable, not reportable"
   },
   {
    "metric": "dominant freq (Hz)",
    "segment": "whole recording",
    "value": "56\u2013107",
    "unit": "Hz",
    "status": "unstable, not reportable"
   },
   {
    "metric": "persistent rotors",
    "segment": "whole recording",
    "value": "1\u20135.21e+03",
    "unit": "count",
    "status": "unstable, not reportable"
   },
   {
    "metric": "singularity frames fraction",
    "segment": "whole recording",
    "value": "1",
    "unit": "fraction of frames",
    "status": "no cited range"
   },
   {
    "metric": "persistent rotor count max",
    "segment": "whole recording",
    "value": "1\u2013124",
    "unit": "count",
    "status": "unstable, not reportable"
   }
  ],
  "verdict": {
   "physiology_ok": null,
   "send_back": false,
   "notes": "Verdict: no physiology pass/fail can be computed because no stable ranged checks are available; the provisional ranges, stability threshold, and criteria should be treated as provisional. What is reportable is the stable whole-recording singularity-frames fraction, range 1.0 to 1.0, only as an observed output without a cited physiological range. What is not reportable as single values is persistent rotors, range 1.0 to 5211.0; maximum concurrent persistent-rotor count, range 1.0 to 124.0; dominant frequency, range 55.989 to 107.311 Hz; and persistent rotor tracks, range 1.0 to 5211.0. A same-species mouse spiral-wave context passage is available, but dominant-frequency comparison should wait because this metric is unstable (DOI 10.1371/journal.pcbi.1011660). Review the tracker count sensitivity, with strict/default/loose counts of 327, 1118, and 94, and the inspector concerns about 977.0 Hz MiCAM frame-rate provenance, likely pre-deposit sign or conditioning, all-negative integer data values, rhythm-detector unsuitability for the expected paced period, and the non-biological mask."
  },
  "provenance": {
   "recording": {
    "id": "ae77081e-a125-471f-8805-27dad0688848",
    "label": "Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_MiCAM",
    "source": "admission",
    "source_ref": "10.5281/zenodo.5557829",
    "publication_ref": "10.1371/journal.pone.0259174",
    "storage_path": "uploads/public/7764050e/mouse_2609_150ms_control_MiCAM.mat",
    "format": "mat",
    "sha256": "6f7fd1ea3f1f2610645c5d235dd4bc189bcdf58c1a038c8f8e68e2233948253e",
    "size_bytes": 30452226,
    "license": "cc-by-4.0",
    "attribution": "Rybashlykov Dmitry",
    "rights_source": {
     "ref": "10.5281/zenodo.5557829",
     "kind": "deposit_metadata",
     "admitted_by": "project lead",
     "license_as_stated": "cc-by-4.0"
    },
    "frame_rate_hz": 977.0,
    "frame_rate_source": {
     "by": "project lead",
     "ref": "10.1371/journal.pone.0259174",
     "kind": "admission",
     "quote": "The custom software was designed to make recordings with a resolution of 200x200 pixels and a framerate of 977 frames per second (FPS).",
     "source": "paper_methods"
    },
    "pixel_size_um": null,
    "pixel_size_source": null,
    "frames": 2047,
    "height": 100,
    "width": 100,
    "inverted": false
   },
   "protocol": {
    "id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
    "name": "Mouse whole heart optical mapping (Luther lab)",
    "species": "mouse",
    "tissue": "whole_heart",
    "params_version": 3,
    "params_set_by": "developer (not a domain scientist)",
    "params_note": "version 3: baseline 901 frames = 2.1 x the 433-frame (2.31 Hz) post-termination cycle resolved under v2; v2's 641 frames was 1.48 cycles, on the 1.5-cycle line, and the pipeline check held one run and passed another on the same inputs. pad 500 >= half the window. Provisional (REVIEW_NEEDED item 16)."
   },
   "run": {
    "id": "6e21b7f8-7cd1-499c-8e8f-ab37b36c7113",
    "status": "done",
    "started_at": "2026-10-28T18:15:27.73036+00:00",
    "finished_at": "2026-10-28T18:18:33.810391+00:00",
    "code": {
     "dirty": true,
     "git_commit": "008c2dc076bcebf9e7fa01a05e8b7c4b5a713b37"
    },
    "workers": 9,
    "steps": [
     "load",
     "deposit_checks",
     "baseline_correct",
     "polarity",
     "spatial_filter(phase only)",
     "hilbert_phase",
     "phase_singularities",
     "track_and_pair",
     "beats_apd_cv",
     "sensitivity",
     "render"
    ],
    "protocol_params": {
     "params": {
      "link_px": 4.0,
      "max_gap": 5,
      "min_cycles": 1.0,
      "pad_frames": 500,
      "paired_frac": 0.5,
      "render_step": 4,
      "pair_dist_px": 3.0,
      "spatial_sigma": 1.0,
      "rotor_erode_px": 3,
      "baseline_frames": 901,
      "rotor_threshold": 0.9,
      "spatial_truncate": 4.0
     },
     "version": 3,
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674"
    },
    "baseline_rule": {
     "ok": true,
     "ratio": 128.714,
     "cycles": {
      "whole_recording": {
       "n_frames": 2047,
       "cycle_frames": 7,
       "pixels_agreeing_frac": 0.8584858485848584,
       "pixels_with_rhythm_frac": 1.0,
       "interval_quartiles_frames": [
        6.0,
        7.0,
        7.0
       ]
      }
     },
     "factor": 2.0,
     "reason": null,
     "source": "per-pixel dominant frequency (images)",
     "status": "provisional",
     "baseline_frames": 901,
     "slowest_segment": "whole_recording",
     "required_min_frames": 14,
     "slowest_cycle_frames": 7
    },
    "deposit_checks": {
     "flags": [
      {
       "flag": "negative_values",
       "value": 1.0,
       "suggests": "baseline-subtracted or normalized; raw fluorescence counts are non-negative",
       "threshold": 0.01
      }
     ],
     "status": "provisional",
     "measures": {
      "max": -25.0,
      "min": -13413.0,
      "frames_used": 2000,
      "integer_frac": 1.0,
      "negative_frac": 1.0,
      "frames_with_nan": 0,
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      "neighbour_dv_corr_median": 0.06412793593881308
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     "processed_before_deposit": true
    },
    "pipeline_check": {
     "model": "openai:gpt-5.5",
     "proceed": true,
     "anomalies": [
      {
       "topic": "Frame-rate provenance for a MiCAM file",
       "severity": "warn",
       "effect_on_analysis": "The fixed pipeline can run with the admitted working frame rate of 977.0 Hz, but the provenance is indirect for this MiCAM, 100x100 deposited file because the cited paper statement is for the custom iDS system and 200x200 acquisition. Time-based interpretation should therefore cite this caveat."
      },
      {
       "topic": "Pre-deposit sign/conditioning",
       "severity": "warn",
       "effect_on_analysis": "The deposited signal appears non-inverted despite the paper naming Di-4-ANEPPS, which normally dims on depolarization. The downstream pipeline orients recordings from the measurement, so this does not by itself invalidate analysis, but polarity and any sign-dependent interpretation should be treated as pre-conditioned before deposit."
      },
      {
       "topic": "Unexplained all-negative data values",
       "severity": "warn",
       "effect_on_analysis": "All sampled values are negative integers, suggesting baseline subtraction, normalization, or another file-level transformation before deposit. This means amplitude baselines and noise estimates may not reflect raw fluorescence counts, and the protocol's analysis is applied to a processed deposited array."
      },
      {
       "topic": "Mask is not a heart segmentation",
       "severity": "warn",
       "effect_on_analysis": "The analysis mask covers nearly the whole image and is based only on nonzero temporal standard deviation, not a biological heart segmentation. Spatial maps from the fixed pipeline may include non-tissue/background pixels unless a biological mask is generated for interpretation outside this run."
      },
      {
       "topic": "Rhythm detector not suitable for the expected paced period here",
       "severity": "info",
       "effect_on_analysis": "The short dominant cycles reported by rhythm tools should not be used as physiology or for frame-rate inference because the expected paced period from the file label and paper is outside the rhythm tool's baseline-correction window; this is a reporting caveat and not a hold condition."
      },
      {
       "topic": "baseline-window rule",
       "severity": "info",
       "effect_on_analysis": "The coded baseline-window rule passed: baseline_frames 901 is at least 2.0 times the slowest coded cycle, with ratio 128.714. Per policy this rule is reported only as informational and does not change proceed status here."
      }
     ],
     "rationale": "The recording may be analysed under the fixed provisional mouse whole-heart protocol as it stands. The protocol does not specify a conflicting fixed indicator, pixel size, frame rate, or image size, and the admitted 977.0 Hz frame rate is the working value on record for this file, although its MiCAM-specific provenance is only medium confidence. The main limitations are not parameter mismatches but provenance and preprocessing warnings: the deposited array appears sign/conditioning transformed, all-negative, and masked nearly as the full image, so the fixed parameters can be applied but results must be interpreted as applying to the processed deposited recording rather than raw fluorescence or a tissue-only mask.",
     "mismatches": [],
     "hold_reason": null,
     "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
     "methods_text": "Using provisional protocol f65d786f-f194-4be2-88b8-10d3dd25d674 with provisional parameter set version 3, the fixed analysis parameters were baseline_frames 901, spatial_sigma 1.0, spatial_truncate 4.0, pad_frames 500, rotor_threshold 0.9, rotor_erode_px 3, link_px 4.0, max_gap 5, min_cycles 1.0, pair_dist_px 3.0, paired_frac 0.5, and render_step 4. The recording has 2047 frames of 100 by 100 pixels in mat format; the frame rate used was 977.0 Hz from the recording-row admission entry citing 10.1371/journal.pone.0259174, and the pixel size was not available. The analysed window used for rhythm reporting was frames 500 to 1547, and the provisional segment definition was one whole_recording segment spanning frames 0 to 2047; the parameter set, segment definitions, and criteria are provisional.",
     "recording_id": "ae77081e-a125-471f-8805-27dad0688848",
     "params_version": 3,
     "prompt_version": "pipeline-v2"
    }
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     "voltage": "runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/voltage.npy",
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     "map_units": {
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     "mask": "not NaN in frame 0 and nonzero temporal std",
     "render": {
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      "palettes": {
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       "quote": "The custom software was designed to make recordings with a resolution of 200x200 pixels and a framerate of 977 frames per second (FPS).",
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     "baseline_method": "scipy.ndimage.uniform_filter1d, mode=reflect, subtracted per pixel",
     "polarity_method": "sign of median per-pixel skew of dV/dt on baseline-corrected signal",
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     "spatial_applies_to": "phase/activation only; displayed voltage is baseline-corrected only"
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       "id": "protocol_parameter_set",
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        "protocol": "Mouse whole heart optical mapping (Luther lab)",
        "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
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       "decision": null,
       "location": "worker/voltage/protocol_params.py (developer_defaults, set_params)",
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       "reviewer": null,
       "description": "Each protocol's fixed, versioned analysis parameters (version 1 = developer defaults); no run or agent may deviate",
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      {
       "id": "baseline_window_rule",
       "expert": "optical mapping analyst",
       "status": "provisional",
       "values": {
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        "source": "per-pixel dominant frequency (images)",
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        "slowest_cycle_frames": 7
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       "location": "worker/voltage/baseline_rule.py (FACTOR, evaluate)",
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       "reviewer": null,
       "description": "A run proceeds only if the protocol's baseline window is >= 2x the slowest segment's cycle, the cycle from the MAP channel when a DAQ log exists, else from per-pixel dominant frequency on linearly detrended pixels; otherwise the run is held by code",
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       "expert": "optical mapping analyst (cardiac electrophysiology)",
       "status": "provisional",
       "values": {
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        "baseline_frames": 901,
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       "decision": null,
       "location": "worker/voltage/preprocess.py (Params)",
       "reviewed": false,
       "reviewer": null,
       "description": "Default baseline window (frames) and spatial Gaussian sigma/truncate for phase",
       "reviewed_at": null
      },
      {
       "id": "tissue_mask",
       "expert": "optical mapping analyst",
       "status": "provisional",
       "values": {
        "rule": "not NaN in frame 0 and nonzero std",
        "tissue_pixels": 9999
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       "decision": null,
       "location": "worker/voltage/io.py (tissue_mask)",
       "reviewed": false,
       "reviewer": null,
       "description": "Tissue = not NaN in frame 0 and nonzero temporal std",
       "reviewed_at": null
      },
      {
       "id": "polarity",
       "expert": "optical mapping analyst / voltage dye chemist",
       "status": "provisional",
       "values": {
        "method": "median skew of dV/dt",
        "result": 1
       },
       "decision": null,
       "location": "worker/voltage/preprocess.py (polarity)",
       "reviewed": false,
       "reviewer": null,
       "description": "Dye polarity from sign of median per-pixel skew of dV/dt",
       "reviewed_at": null
      },
      {
       "id": "singularity_detection",
       "expert": "computational cardiac electrophysiologist (phase mapping)",
       "status": "provisional",
       "values": {
        "rotor_erode_px": 3,
        "rotor_threshold": 0.9
       },
       "decision": null,
       "location": "worker/voltage/preprocess.py (Params.rotor_threshold, rotor_erode_px)",
       "reviewed": false,
       "reviewer": null,
       "description": "Phase singularity = |winding| > threshold turns, >= erode px from tissue edge",
       "reviewed_at": null
      },
      {
       "id": "rotor_persistence",
       "expert": "computational cardiac electrophysiologist (phase mapping)",
       "status": "provisional",
       "values": {
        "link_px": 4.0,
        "max_gap": 5,
        "min_cycles": 1.0,
        "min_frames": 17
       },
       "decision": null,
       "location": "worker/voltage/rotors.py (TrackParams)",
       "reviewed": false,
       "reviewer": null,
       "description": "Track linking distance/gap and minimum lifetime for a persistent rotor",
       "reviewed_at": null
      },
      {
       "id": "chirality_pairing",
       "expert": "computational cardiac electrophysiologist (phase mapping)",
       "status": "provisional",
       "values": {
        "paired_frac": 0.5,
        "pair_dist_px": 3.0
       },
       "decision": null,
       "location": "worker/voltage/rotors.py (TrackParams.pair_dist_px, paired_frac)",
       "reviewed": false,
       "reviewer": null,
       "description": "Opposite-charge singularities within pair distance are a pair; tracks paired most of their life are excluded from rotors",
       "reviewed_at": null
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      {
       "id": "sensitivity_grid",
       "expert": "optical mapping analyst",
       "status": "provisional",
       "values": {
        "sigmas": {
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         "default": 1.0,
         "heavier": 2.0
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        "trackers": {
         "loose": [
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         "strict": [
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         "default": [
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         "3x_cycle": 51,
         "6x_cycle": 102,
         "1.5x_cycle": 26
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       "decision": null,
       "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
       "reviewed": false,
       "reviewer": null,
       "description": "Filter levels tested: spatial sigma and baseline window values",
       "reviewed_at": null
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      {
       "id": "analysis_window",
       "expert": "cardiac electrophysiologist familiar with the dataset",
       "status": "provisional",
       "values": {
        "f0": 500,
        "f1": 1547
       },
       "decision": null,
       "location": "worker/voltage/runs.py (default_window)",
       "reviewed": false,
       "reviewer": null,
       "description": "Frames analysed: whole recording minus one filter pad at each end (unless the plan narrows it)",
       "reviewed_at": null
      },
      {
       "id": "prior_processing_flags",
       "expert": "imaging scientist (camera acquisition and noise)",
       "status": "provisional",
       "values": {
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        "thresholds": {
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         "neg_frac": 0.01,
         "dv_autocorr": 0.5,
         "neighbour_corr": 0.8
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       },
       "decision": null,
       "location": "worker/voltage/inspect.py (prior_processing)",
       "reviewed": false,
       "reviewer": null,
       "description": "Thresholds for flagging depositor processing (normalization, masking, temporal/spatial smoothing)",
       "reviewed_at": null
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       "id": "pipeline_check",
       "expert": "optical mapping analyst",
       "status": "provisional",
       "values": {
        "model": "openai:gpt-5.5",
        "proceed": true,
        "anomalies": [
         "Frame-rate provenance for a MiCAM file",
         "Pre-deposit sign/conditioning",
         "Unexplained all-negative data values",
         "Mask is not a heart segmentation",
         "Rhythm detector not suitable for the expected paced period here",
         "baseline-window rule"
        ],
        "mismatches": []
       },
       "decision": null,
       "location": "worker/agents/pipeline.py (INSTRUCTIONS, validator)",
       "reviewed": false,
       "reviewer": null,
       "description": "The pipeline agent's hold/proceed decision from protocol mismatches and inspector anomalies; it chooses no parameter",
       "reviewed_at": null
      },
      {
       "id": "physiology_reference_rates",
       "expert": "cardiac electrophysiologist per species",
       "status": "provisional",
       "values": {
        "ranges": [
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        ],
        "tolerance_frac": 0.25,
        "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
       },
       "decision": null,
       "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
       "reviewed": false,
       "reviewer": null,
       "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
       "reviewed_at": null
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      {
       "id": "stability_rule",
       "expert": "biostatistician + optical mapping analyst",
       "status": "provisional",
       "values": {
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         "whole_recording.persistent_rotor_count_max",
         "whole_recording.dominant_freq_hz",
         "persistent_rotor_tracks"
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        "stable_frac": 0.25
       },
       "decision": null,
       "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
       "reviewed": false,
       "reviewer": null,
       "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
       "reviewed_at": null
      },
      {
       "id": "persistence_threshold_variants",
       "expert": "computational cardiac electrophysiologist (phase mapping)",
       "status": "provisional",
       "values": {
        "measured_cycle": 17,
        "paper_rotation": null
       },
       "decision": null,
       "location": "worker/agents/critic.py (persistence_variants)",
       "reviewed": false,
       "reviewer": null,
       "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
       "reviewed_at": null
      },
      {
       "id": "persistent_rotor_criterion",
       "expert": "cardiac electrophysiologist",
       "status": "provisional",
       "values": {
        "max_absent": 0,
        "min_present": 1
       },
       "decision": null,
       "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
       "reviewed": false,
       "reviewer": null,
       "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
       "reviewed_at": null
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     "all_reviewed": false,
     "superseded_by_run": null
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     "definition": "whole recording; no events detected or logged at admission",
     "defined_by": "admission (project lead); no comparison defined",
     "citation": null,
     "evidence": {},
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    "baseline_correct",
    "polarity",
    "spatial_filter(phase only)",
    "hilbert_phase",
    "phase_singularities",
    "track_and_pair",
    "beats_apd_cv",
    "sensitivity",
    "render"
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   "protocol_params": {
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    "proceed": true,
    "anomalies": [
     {
      "topic": "Frame-rate provenance for a MiCAM file",
      "severity": "warn",
      "effect_on_analysis": "The fixed pipeline can run with the admitted working frame rate of 977.0 Hz, but the provenance is indirect for this MiCAM, 100x100 deposited file because the cited paper statement is for the custom iDS system and 200x200 acquisition. Time-based interpretation should therefore cite this caveat."
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     {
      "topic": "Pre-deposit sign/conditioning",
      "severity": "warn",
      "effect_on_analysis": "The deposited signal appears non-inverted despite the paper naming Di-4-ANEPPS, which normally dims on depolarization. The downstream pipeline orients recordings from the measurement, so this does not by itself invalidate analysis, but polarity and any sign-dependent interpretation should be treated as pre-conditioned before deposit."
     },
     {
      "topic": "Unexplained all-negative data values",
      "severity": "warn",
      "effect_on_analysis": "All sampled values are negative integers, suggesting baseline subtraction, normalization, or another file-level transformation before deposit. This means amplitude baselines and noise estimates may not reflect raw fluorescence counts, and the protocol's analysis is applied to a processed deposited array."
     },
     {
      "topic": "Mask is not a heart segmentation",
      "severity": "warn",
      "effect_on_analysis": "The analysis mask covers nearly the whole image and is based only on nonzero temporal standard deviation, not a biological heart segmentation. Spatial maps from the fixed pipeline may include non-tissue/background pixels unless a biological mask is generated for interpretation outside this run."
     },
     {
      "topic": "Rhythm detector not suitable for the expected paced period here",
      "severity": "info",
      "effect_on_analysis": "The short dominant cycles reported by rhythm tools should not be used as physiology or for frame-rate inference because the expected paced period from the file label and paper is outside the rhythm tool's baseline-correction window; this is a reporting caveat and not a hold condition."
     },
     {
      "topic": "baseline-window rule",
      "severity": "info",
      "effect_on_analysis": "The coded baseline-window rule passed: baseline_frames 901 is at least 2.0 times the slowest coded cycle, with ratio 128.714. Per policy this rule is reported only as informational and does not change proceed status here."
     }
    ],
    "rationale": "The recording may be analysed under the fixed provisional mouse whole-heart protocol as it stands. The protocol does not specify a conflicting fixed indicator, pixel size, frame rate, or image size, and the admitted 977.0 Hz frame rate is the working value on record for this file, although its MiCAM-specific provenance is only medium confidence. The main limitations are not parameter mismatches but provenance and preprocessing warnings: the deposited array appears sign/conditioning transformed, all-negative, and masked nearly as the full image, so the fixed parameters can be applied but results must be interpreted as applying to the processed deposited recording rather than raw fluorescence or a tissue-only mask.",
    "mismatches": [],
    "hold_reason": null,
    "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
    "methods_text": "Using provisional protocol f65d786f-f194-4be2-88b8-10d3dd25d674 with provisional parameter set version 3, the fixed analysis parameters were baseline_frames 901, spatial_sigma 1.0, spatial_truncate 4.0, pad_frames 500, rotor_threshold 0.9, rotor_erode_px 3, link_px 4.0, max_gap 5, min_cycles 1.0, pair_dist_px 3.0, paired_frac 0.5, and render_step 4. The recording has 2047 frames of 100 by 100 pixels in mat format; the frame rate used was 977.0 Hz from the recording-row admission entry citing 10.1371/journal.pone.0259174, and the pixel size was not available. The analysed window used for rhythm reporting was frames 500 to 1547, and the provisional segment definition was one whole_recording segment spanning frames 0 to 2047; the parameter set, segment definitions, and criteria are provisional.",
    "recording_id": "ae77081e-a125-471f-8805-27dad0688848",
    "params_version": 3,
    "prompt_version": "pipeline-v2"
   }
  },
  "signature": {
   "id": "804ddd67-f7b9-45c6-a823-bef795065584",
   "created_at": "2026-10-28T18:17:28.845214+00:00",
   "movie_path": "runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/movie.mp4",
   "frames": {
    "f0": 500,
    "f1": 1547,
    "maps": "runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/maps.npz",
    "dtype": "<f4",
    "phase": "runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/phase.npy",
    "shape": [
     1047,
     100,
     100
    ],
    "sha256": {
     "maps": "a5d54d086a101f4dd713d94cdd909059b29b9027f0fd8dd9c9e4544afc26d5b6",
     "phase": "a7f991a270140f9a40c42b847371848291c872a97ebdce5a5cbf5eae065f1307",
     "voltage": "35e7b15db730a09c2f8db8fff35d6aa572a4acf7b39fbf317758c5e400c7762d"
    },
    "voltage": "runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/voltage.npy",
    "map_keys": [
     "activation:whole_recording",
     "dominant_frequency:whole_recording",
     "dominant_frequency:window"
    ],
    "map_units": {
     "dominant_frequency:window": "Hz",
     "activation:whole_recording": "ms",
     "dominant_frequency:whole_recording": "Hz"
    }
   },
   "filter_params": {
    "mask": "not NaN in frame 0 and nonzero temporal std",
    "render": {
     "step": 4,
     "palettes": {
      "phase": "twilight",
      "camera": "gray",
      "voltage": "gray"
     },
     "interpolation": "nearest"
    },
    "window": {
     "f0": 500,
     "f1": 1547,
     "segments": [
      {
       "name": "whole_recording",
       "label": "whole recording",
       "defined": [
        0,
        2047
       ],
       "analysed": [
        500,
        1547
       ],
       "citation": null,
       "defined_by": "admission (project lead); no comparison defined",
       "segment_id": "f43dd23d-fc9d-4a02-a299-3c478800b938",
       "end_event_id": null,
       "start_event_id": null,
       "expected_persistent_rotors": null
      }
     ],
     "illumination": null,
     "frame_rate_hz": 977.0,
     "frame_rate_source": {
      "by": "project lead",
      "ref": "10.1371/journal.pone.0259174",
      "kind": "admission",
      "quote": "The custom software was designed to make recordings with a resolution of 200x200 pixels and a framerate of 977 frames per second (FPS).",
      "source": "paper_methods"
     },
     "segment_cycles_frames": {
      "whole_recording": 17
     }
    },
    "lowpass": null,
    "polarity": 1,
    "tracking": {
     "link_px": 4.0,
     "max_gap": 5,
     "min_cycles": 1.0,
     "min_frames": 17,
     "paired_frac": 0.5,
     "pair_dist_px": 3.0
    },
    "pad_frames": 500,
    "phase_method": "angle of scipy.signal.hilbert along time, on spatially filtered signal",
    "spatial_sigma": 1.0,
    "rotor_erode_px": 3,
    "spatial_method": "scipy.ndimage.gaussian_filter per frame, mode=reflect; NaN set to 0 before filtering, non-tissue zeroed after",
    "baseline_frames": 901,
    "baseline_method": "scipy.ndimage.uniform_filter1d, mode=reflect, subtracted per pixel",
    "polarity_method": "sign of median per-pixel skew of dV/dt on baseline-corrected signal",
    "rotor_threshold": 0.9,
    "spatial_truncate": 4.0,
    "spatial_applies_to": "phase/activation only; displayed voltage is baseline-corrected only"
   },
   "criteria_status": {
    "note": null,
    "rules": [
     {
      "id": "protocol_parameter_set",
      "expert": "optical mapping analyst",
      "status": "provisional",
      "values": {
       "set_by": "developer (not a domain scientist)",
       "protocol": "Mouse whole heart optical mapping (Luther lab)",
       "protocol_id": "f65d786f-f194-4be2-88b8-10d3dd25d674",
       "params_version": 3
      },
      "decision": null,
      "location": "worker/voltage/protocol_params.py (developer_defaults, set_params)",
      "reviewed": false,
      "reviewer": null,
      "description": "Each protocol's fixed, versioned analysis parameters (version 1 = developer defaults); no run or agent may deviate",
      "reviewed_at": null
     },
     {
      "id": "baseline_window_rule",
      "expert": "optical mapping analyst",
      "status": "provisional",
      "values": {
       "ok": true,
       "factor": 2.0,
       "source": "per-pixel dominant frequency (images)",
       "required_min_frames": 14,
       "slowest_cycle_frames": 7
      },
      "decision": null,
      "location": "worker/voltage/baseline_rule.py (FACTOR, evaluate)",
      "reviewed": false,
      "reviewer": null,
      "description": "A run proceeds only if the protocol's baseline window is >= 2x the slowest segment's cycle, the cycle from the MAP channel when a DAQ log exists, else from per-pixel dominant frequency on linearly detrended pixels; otherwise the run is held by code",
      "reviewed_at": null
     },
     {
      "id": "filter_defaults",
      "expert": "optical mapping analyst (cardiac electrophysiology)",
      "status": "provisional",
      "values": {
       "lowpass": null,
       "spatial_sigma": 1.0,
       "baseline_frames": 901,
       "spatial_truncate": 4.0
      },
      "decision": null,
      "location": "worker/voltage/preprocess.py (Params)",
      "reviewed": false,
      "reviewer": null,
      "description": "Default baseline window (frames) and spatial Gaussian sigma/truncate for phase",
      "reviewed_at": null
     },
     {
      "id": "tissue_mask",
      "expert": "optical mapping analyst",
      "status": "provisional",
      "values": {
       "rule": "not NaN in frame 0 and nonzero std",
       "tissue_pixels": 9999
      },
      "decision": null,
      "location": "worker/voltage/io.py (tissue_mask)",
      "reviewed": false,
      "reviewer": null,
      "description": "Tissue = not NaN in frame 0 and nonzero temporal std",
      "reviewed_at": null
     },
     {
      "id": "polarity",
      "expert": "optical mapping analyst / voltage dye chemist",
      "status": "provisional",
      "values": {
       "method": "median skew of dV/dt",
       "result": 1
      },
      "decision": null,
      "location": "worker/voltage/preprocess.py (polarity)",
      "reviewed": false,
      "reviewer": null,
      "description": "Dye polarity from sign of median per-pixel skew of dV/dt",
      "reviewed_at": null
     },
     {
      "id": "singularity_detection",
      "expert": "computational cardiac electrophysiologist (phase mapping)",
      "status": "provisional",
      "values": {
       "rotor_erode_px": 3,
       "rotor_threshold": 0.9
      },
      "decision": null,
      "location": "worker/voltage/preprocess.py (Params.rotor_threshold, rotor_erode_px)",
      "reviewed": false,
      "reviewer": null,
      "description": "Phase singularity = |winding| > threshold turns, >= erode px from tissue edge",
      "reviewed_at": null
     },
     {
      "id": "rotor_persistence",
      "expert": "computational cardiac electrophysiologist (phase mapping)",
      "status": "provisional",
      "values": {
       "link_px": 4.0,
       "max_gap": 5,
       "min_cycles": 1.0,
       "min_frames": 17
      },
      "decision": null,
      "location": "worker/voltage/rotors.py (TrackParams)",
      "reviewed": false,
      "reviewer": null,
      "description": "Track linking distance/gap and minimum lifetime for a persistent rotor",
      "reviewed_at": null
     },
     {
      "id": "chirality_pairing",
      "expert": "computational cardiac electrophysiologist (phase mapping)",
      "status": "provisional",
      "values": {
       "paired_frac": 0.5,
       "pair_dist_px": 3.0
      },
      "decision": null,
      "location": "worker/voltage/rotors.py (TrackParams.pair_dist_px, paired_frac)",
      "reviewed": false,
      "reviewer": null,
      "description": "Opposite-charge singularities within pair distance are a pair; tracks paired most of their life are excluded from rotors",
      "reviewed_at": null
     },
     {
      "id": "sensitivity_grid",
      "expert": "optical mapping analyst",
      "status": "provisional",
      "values": {
       "sigmas": {
        "light": 0.5,
        "default": 1.0,
        "heavier": 2.0
       },
       "trackers": {
        "loose": [
         6.0,
         10
        ],
        "strict": [
         2.0,
         1
        ],
        "default": [
         4.0,
         5
        ]
       },
       "baselines": {
        "run": 901,
        "3x_cycle": 51,
        "6x_cycle": 102,
        "1.5x_cycle": 26
       }
      },
      "decision": null,
      "location": "worker/voltage/sensitivity.py (SIGMAS, BASELINE_MULTIPLES, baseline_levels)",
      "reviewed": false,
      "reviewer": null,
      "description": "Filter levels tested: spatial sigma and baseline window values",
      "reviewed_at": null
     },
     {
      "id": "analysis_window",
      "expert": "cardiac electrophysiologist familiar with the dataset",
      "status": "provisional",
      "values": {
       "f0": 500,
       "f1": 1547
      },
      "decision": null,
      "location": "worker/voltage/runs.py (default_window)",
      "reviewed": false,
      "reviewer": null,
      "description": "Frames analysed: whole recording minus one filter pad at each end (unless the plan narrows it)",
      "reviewed_at": null
     },
     {
      "id": "prior_processing_flags",
      "expert": "imaging scientist (camera acquisition and noise)",
      "status": "provisional",
      "values": {
       "flags": [
        "negative_values"
       ],
       "thresholds": {
        "int_frac": 0.5,
        "neg_frac": 0.01,
        "dv_autocorr": 0.5,
        "neighbour_corr": 0.8
       }
      },
      "decision": null,
      "location": "worker/voltage/inspect.py (prior_processing)",
      "reviewed": false,
      "reviewer": null,
      "description": "Thresholds for flagging depositor processing (normalization, masking, temporal/spatial smoothing)",
      "reviewed_at": null
     },
     {
      "id": "pipeline_check",
      "expert": "optical mapping analyst",
      "status": "provisional",
      "values": {
       "model": "openai:gpt-5.5",
       "proceed": true,
       "anomalies": [
        "Frame-rate provenance for a MiCAM file",
        "Pre-deposit sign/conditioning",
        "Unexplained all-negative data values",
        "Mask is not a heart segmentation",
        "Rhythm detector not suitable for the expected paced period here",
        "baseline-window rule"
       ],
       "mismatches": []
      },
      "decision": null,
      "location": "worker/agents/pipeline.py (INSTRUCTIONS, validator)",
      "reviewed": false,
      "reviewer": null,
      "description": "The pipeline agent's hold/proceed decision from protocol mismatches and inspector anomalies; it chooses no parameter",
      "reviewed_at": null
     },
     {
      "id": "physiology_reference_rates",
      "expert": "cardiac electrophysiologist per species",
      "status": "provisional",
      "values": {
       "ranges": [
        "mouse_spiral_rotation_hz"
       ],
       "tolerance_frac": 0.25,
       "segment_rhythm_rule": "expected persistent rotors 'present' -> spiral arrhythmia"
      },
      "decision": null,
      "location": "worker/voltage/physiology.py (RANGES, TOLERANCE_FRAC)",
      "reviewed": false,
      "reviewer": null,
      "description": "Cited physiological ranges (single-study values widened by a 25% tolerance), applied to segments by their expected rhythm; used by the critic and for frame-rate sanity checks",
      "reviewed_at": null
     },
     {
      "id": "stability_rule",
      "expert": "biostatistician + optical mapping analyst",
      "status": "provisional",
      "values": {
       "unstable": [
        "whole_recording.persistent_rotors",
        "whole_recording.persistent_rotor_count_max",
        "whole_recording.dominant_freq_hz",
        "persistent_rotor_tracks"
       ],
       "stable_frac": 0.25
      },
      "decision": null,
      "location": "worker/agents/critic.py (STABLE_FRAC, stability)",
      "reviewed": false,
      "reviewer": null,
      "description": "A metric is reportable only if its spread across all sensitivity settings is <= 25% of its median (zero spread when all values are 0); otherwise 'unstable, not reportable'",
      "reviewed_at": null
     },
     {
      "id": "persistence_threshold_variants",
      "expert": "computational cardiac electrophysiologist (phase mapping)",
      "status": "provisional",
      "values": {
       "measured_cycle": 17,
       "paper_rotation": null
      },
      "decision": null,
      "location": "worker/agents/critic.py (persistence_variants)",
      "reviewed": false,
      "reviewer": null,
      "description": "The persistent-rotor rule is run at two lifetimes, one measured cycle of the fastest segment and the paper's cited rotation period; both verdicts stored, disagreement flagged",
      "reviewed_at": null
     },
     {
      "id": "persistent_rotor_criterion",
      "expert": "cardiac electrophysiologist",
      "status": "provisional",
      "values": {
       "max_absent": 0,
       "min_present": 1
      },
      "decision": null,
      "location": "worker/voltage/sensitivity.py (MIN_ROTORS_PRESENT, MAX_ROTORS_ABSENT)",
      "reviewed": false,
      "reviewer": null,
      "description": "Persistent-rotor finding holds if every 'present' segment has >= 1 persistent rotor and every 'absent' segment has none, at every setting",
      "reviewed_at": null
     }
    ],
    "status": "provisional",
    "all_reviewed": false,
    "superseded_by_run": null
   }
  },
  "segments": [
   {
    "id": "f43dd23d-fc9d-4a02-a299-3c478800b938",
    "position": 1,
    "name": "whole_recording",
    "label": "whole recording",
    "start_frame": 0,
    "end_frame": 2047,
    "expected_persistent_rotors": null,
    "definition": "whole recording; no events detected or logged at admission",
    "defined_by": "admission (project lead); no comparison defined",
    "citation": null,
    "evidence": {},
    "provisional": true
   }
  ],
  "events": []
 }
}