SignatureRun

mouse_2609_150ms_control_MiCAM, run 2aec1b88

Status done, stage done; requested by project lead; worker local worker; started 2026-10-28 18:59 UTC, finished 2026-10-28 19:01 UTC, 147 s; signature 0187eb93

Stages

StageStartedSeconds
claimed18:58:590.6
pipeline_check18:59:0023.9
baseline_rule18:59:240.5
deposit_checks18:59:240.2
analysis18:59:257.8
render18:59:3258.6
persist19:00:310.6
critic19:00:3279.0
done19:01:510.1

Log

TimeAgentActionDetail
18:58:58webrun queued{"by": "project lead", "reason": "mask-rule acceptance, rerun of the recording behind 804ddd67", "recording_id": "ae77081e-a125-471f-8805-27dad0688848", "run_id": "2aec1b88-1bc3-430f-9d57-a9ddcf39928c"}
18:59:24runsrun started{"checked_by": "openai:gpt-5.5", "protocol_params_version": 3, "recording_id": "ae77081e-a125-471f-8805-27dad0688848", "run_id": "2aec1b88-1bc3-430f-9d57-a9ddcf39928c"}
19:00:32runssignature written{"criteria": "provisional", "holds": null, "run_id": "2aec1b88-1bc3-430f-9d57-a9ddcf39928c", "signature_id": "0187eb93-2c8b-4500-b11c-9d0062c96d4a"}

Entries from the audit log for this run; new rows and stage changes arrive by realtime while the run is open (connecting).

Pipeline check

Proceed: True; parameters v3.

FieldProtocolRecordingSeverityDetail
no mismatches
AnomalySeverityEffect on the Fixed Pipeline
Frame-rate provenance for a MiCAM filewarnThe run should use 977.0 Hz from the recording-row admission source, but the cited paper text refers to the custom/iDS system and 200x200 acquisition while this deposited file is labelled MiCAM and is 100x100. Frame-based protocol parameters are unchanged; any time conversion or rate interpretation should note the medium-confidence provenance.
Pre-deposit sign/conditioningwarnThe measured polarity is consistently non-inverted although Di-4-ANEPPS normally dims on depolarization, implying a sign flip or other transformation before deposit/export. The fixed pipeline orients from the measurement, so this does not by itself invalidate the run, but polarity-dependent interpretation should be cautious.
Unexplained all-negative data valueswarnAll sampled values are negative integers, suggesting baseline subtraction, normalization, or other processing before deposit. The protocol can still run, but amplitudes, noise estimates, and comparisons to raw fluorescence are affected by undocumented prior processing.
Mask is not a heart segmentationwarnThe analysis mask is based only on nonzero temporal standard deviation and covers 9999/10000 pixels, so spatial maps may include non-tissue/background pixels. Analyses that require tissue-only pixels need a biological mask before interpretation.
baseline-window ruleinfoThe coded baseline rule passed: baseline_frames 901 is at least 2.0 times the slowest segment cycle reported by the rule, with ratio 40.955. This rule is provisional and is reported as informational only.
Rhythm detector not suitable for the expected paced period hereinfoThe inspector cautions that the short-cycle rhythm result used in one detector should not be used to infer physiology or frame rate for this recording. The segment rhythm report for the protocol baseline gives the analysed window and dominant cycle separately; no expected outcome is inferred.

The fixed protocol is appropriate to run as-is because the recording is a mouse-labelled public reference recording under the Luther lab mouse whole-heart optical-mapping protocol, and there are no recording facts that directly conflict with specified protocol fields. The protocol does not specify a required indicator, pixel size, frame rate, or image size, so the available recording facts do not create a blocking mismatch. The main limitations are provenance and preprocessing warnings: the working frame rate is admitted as 977.0 Hz but is supported by paper text for a different named camera/resolution, and the deposited data appear transformed before deposit. These issues affect interpretation and reporting rather than requiring parameter changes under the fixed version-3 protocol.

Inspector

This is a mouse whole-heart MiCAM optical-mapping recording from the Zenodo deposit for the open-source low-cost cardiac optical mapping system paper. Use 977.0 Hz because it is already on the recording row from admission, but note that the supporting paper quote is for 200x200 iDS acquisition while this file is 100x100 MiCAM; the observed 142.9-frame cycle is nevertheless consistent with a ~150 ms paced recording at 977 Hz. The deposited signal is not inverted by measurement—depolarization increases values—although Di-4-ANEPPS normally dims on depolarization, suggesting a pre-deposit sign flip or similar transformation. No acquisition log or detected events are available; the file also appears preprocessed/converted because all values are negative, and that specific transformation is not documented in the deposit metadata.

MeasurementValueMethod, Caveat
SNR, median (p10–p90)15.3 (11.2–20.0)assumes white noise; on temporally smoothed deposits noise is underestimated and SNR inflated
Mask coverage11.1%, 1114 of 10000 pxfile_variable
Polarityblocks 4, inverted False, blocks agreeing 4, median dvdt skew 0.9510276913642883sign of median per-pixel skew of dV/dt, baseline-corrected (121 frames)
Drift / bleachingbaseline change 29.6, vs beat amplitude 0.326, slow range 58.9, trend, Spearman 0.394running median (205 frames) of the tissue-mean raw signal; steps from events (light, rhythm change) and depositor normalization or drift removal change or erase the baseline; bleaching cannot be separated from them here
Prior processingnegative valuesdeposit checks
Dominant cycle by block, frames0–1000: 142.9, 1000–2000: 142.9
ConcernSeverityDetail
Frame-rate provenance for MiCAM filewarnThe on-record frame rate is 977.0 Hz from an admission entry quoting the paper, but the quote describes custom iDS software at 200x200 pixels, whereas this recording is a 100x100 MiCAM file. The 977 Hz value is still preferred by the required source hierarchy, and the 142.9-frame rhythm is consistent with the file label/protocol at 150 ms, but the camera/resolution mismatch should be noted.
Undocumented pre-deposit processing/sign conventionwarnAll sampled values are negative (negative_frac 1.0; min -13413.0, max -25.0), so the file is not raw non-negative camera counts. The deposit metadata does not document the transformation for this file; measured polarity is nevertheless consistent across blocks, so the pipeline can orient it from measurement.
Provisional rhythm/rate referencesinfoThe detected dominant cycle is 142.9 frames in both 1000-frame blocks; conclusions using tool reference rates or thresholds depend on provisional, not yet domain-reviewed, criteria.

Inspector report 2026-10-28 19:16 UTC, model openai:gpt-5.5, provisional. Median per-pixel SNR over the tissue mask is 15.2519519217203, with p10 11.175782496478845 and p90 20.043913618322257 over 2047 frames. The SNR method assumes white noise; if the deposit was temporally smoothed, noise would be underestimated and SNR inflated. The tissue mask contains 1114 pixels out of 10000, coverage 0.1114. It was derived from the file variable bgimage using an Otsu threshold of 2165.203125 and the largest connected component in the class with more temporal signal; there were 0 NaN pixels in frame 0. No acquisition log is present in the deposit, and the stored_events and detected_events tools returned no events for this recording. Therefore there are no logged light transitions or detected image baseline steps to interpret; any pacing interpretation comes only from the label/protocol, not from event detections. The tissue-mean slow baseline changed from -4082.009765625 to -4052.447998046875, a change of 29.561767578125, equal to 0.3261039352774519 of the beat amplitude; the slow baseline range was 58.898681640625 with Spearman trend 0.39378869681589407. The tool cautions that baseline steps, depositor normalization, or drift removal can alter this estimate, and bleaching cannot be separated here.

Range checks

MetricSegmentValue at Run SettingCited RangeStatus
persistent rotor tracksall0 countnone citedunstable not reportable
dominant freq (Hz)whole recording6.532 Hznone citedno cited range
persistent rotorswhole recording0 countnone citedunstable not reportable
singularity frames fractionwhole recording0.4852 fraction of framesnone citedunstable not reportable
persistent rotor count maxwhole recording0 countnone citedunstable not reportable

Compare with run 6e21b7f8

A: this run, code 0aee293 (uncommitted changes), 4 workers. B: run 6e21b7f8, 2026-10-28 18:15 UTC, code 008c2dc (uncommitted changes), 9 workers. 641 stored values differ.

FileABSameHow
frames/voltage833b285fc61635e7b15db730norecorded sha256
frames/phasebbc12dc0da05a7f991a27014norecorded sha256
frames/maps2faef5c48ac4a5d54d086a10norecorded sha256
maps/activation.png8b81398c5734168ec49e8534nosha256 of the stored file
maps/dominant_frequency.pngdf34950269cd52659394f372nosha256 of the stored file
movie.mp46081236673551nosize in bytes only; frames not compared
Stored ValueAB
metrics.amplitude_p99147.6215820312570.9804458618164
metrics.apd50_ms22.5179119754350054.094165813715455
metrics.apd90_ms58.3418628454452355.117707267144319
metrics.dominant_freq6.53199617956064955.98853868194843
metrics.dominant_freq_iqr[1]6.531996179560649297.67239732569243
metrics.mask_coverage0.11140.9999
metrics.min_rotor_frames15017
metrics.paired_singularity_frac0.60982264665757160.6829205217113166
metrics.persistent_rotor_count_max043
metrics.persistent_rotor_durationslength 0length 1118
metrics.persistent_rotor_frames_frac0.01.0
metrics.persistent_rotor_lifetimeslength 0length 1118
metrics.persistent_rotor_tracks01118
metrics.persistent_rotors01118
metrics.segments.whole_recording.beats.apd50_ms_median22.5179119754350054.094165813715455
metrics.segments.whole_recording.beats.apd90_ms_median58.3418628454452355.117707267144319
metrics.segments.whole_recording.beats.beats863
metrics.segments.whole_recording.beats.cv_px_per_frame_median1.58673468699921431.1264385868026237
metrics.segments.whole_recording.beats.global_amplitude_ratio0.75345158957472642.0020047681007953
metrics.segments.whole_recording.beats.per_beatlength 8length 63
metrics.segments.whole_recording.beats.per_beat[0].activation_coverage0.11140.9999
metrics.segments.whole_recording.beats.per_beat[0].activation_spread_frames100.014.0
metrics.segments.whole_recording.beats.per_beat[0].activation_spread_ms102.3541453428863814.329580348004093
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd50.defined_frac0.32675044883303410.6431643164316432
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd50.iqr[0]6.03.0
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd50.iqr[1]38.07.0
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd50.median16.04.0
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd90.defined_frac0.28815080789946140.5549554955495549
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd90.iqr[0]8.03.0
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd90.iqr[1]44.08.0
metrics.segments.whole_recording.beats.per_beat[0].apd_frames.apd90.median20.05.0
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd50.iqr[0]6.14124872057318253.0706243602865912
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd50.iqr[1]38.894575230296827.1647901740020465
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd50.median16.376663254861824.094165813715455
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd90.iqr[0]8.188331627430913.0706243602865912
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd90.iqr[1]45.035823950870018.18833162743091
metrics.segments.whole_recording.beats.per_beat[0].apd_ms.apd90.median20.4708290685772775.117707267144319
metrics.segments.whole_recording.beats.per_beat[0].cv.iqr_px_per_frame[0]0.108065970240949470.7806330180189278
metrics.segments.whole_recording.beats.per_beat[0].cv.iqr_px_per_frame[1]0.232399698121604521.6944783713599114
metrics.segments.whole_recording.beats.per_beat[0].cv.median_px_per_frame0.153845493734597271.1048364779636972
metrics.segments.whole_recording.beats.per_beat[0].cv.pixels_total11149999
metrics.segments.whole_recording.beats.per_beat[0].cv.pixels_used11149975
metrics.segments.whole_recording.beats.per_beat[0].cv.plane_fit.direction_deg97.0638630201104218.654676316148233
metrics.segments.whole_recording.beats.per_beat[0].cv.plane_fit.speed_px_per_frame2.1777025260230816232.25900999717285
metrics.segments.whole_recording.beats.per_beat[0].upstroke_frame524507
metrics.segments.whole_recording.beats.per_beat[0].window[0]500504
metrics.segments.whole_recording.beats.per_beat[0].window[1]610521
metrics.segments.whole_recording.beats.per_beat[1].activation_coverage0.11140.9999
metrics.segments.whole_recording.beats.per_beat[1].activation_spread_frames54.015.0
metrics.segments.whole_recording.beats.per_beat[1].activation_spread_ms55.2712384851586515.353121801432957
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd50.defined_frac0.96858168761220830.6368636863686369
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd50.iqr[0]13.03.0
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd50.iqr[1]27.07.0
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd50.median21.04.0
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd90.defined_frac0.96499102333931770.5481548154815482
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd90.iqr[0]40.03.0
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd90.iqr[1]62.08.0
metrics.segments.whole_recording.beats.per_beat[1].apd_frames.apd90.median57.05.0
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd50.iqr[0]13.3060388945752293.0706243602865912
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd50.iqr[1]27.6356192425793247.1647901740020465
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd50.median21.494370522006144.094165813715455
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd90.iqr[0]40.9416581371545553.0706243602865912
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd90.iqr[1]63.459570112589568.18833162743091
metrics.segments.whole_recording.beats.per_beat[1].apd_ms.apd90.median58.3418628454452355.117707267144319
metrics.segments.whole_recording.beats.per_beat[1].cv.iqr_px_per_frame[0]0.248779997661407630.7723067706932504
metrics.segments.whole_recording.beats.per_beat[1].cv.iqr_px_per_frame[1]3.09840556395811451.6978225489908327
metrics.segments.whole_recording.beats.per_beat[1].cv.median_px_per_frame1.50124418964651851.082078460872887
metrics.segments.whole_recording.beats.per_beat[1].cv.pixels_total11149999
metrics.segments.whole_recording.beats.per_beat[1].cv.pixels_used11119972
metrics.segments.whole_recording.beats.per_beat[1].cv.plane_fit.direction_deg-105.46115931820133-16.72055543576628
metrics.segments.whole_recording.beats.per_beat[1].cv.plane_fit.speed_px_per_frame1.692450978842342429.3599796322268
metrics.segments.whole_recording.beats.per_beat[1].upstroke_frame640524
metrics.segments.whole_recording.beats.per_beat[1].window[0]610521
metrics.segments.whole_recording.beats.per_beat[1].window[1]760538
metrics.segments.whole_recording.beats.per_beat[2].activation_coverage0.11140.9999
metrics.segments.whole_recording.beats.per_beat[2].activation_spread_frames52.6999999999998214.0
metrics.segments.whole_recording.beats.per_beat[2].activation_spread_ms53.94063459570093514.329580348004093
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd50.defined_frac0.97307001795332140.6257625762576258
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd50.iqr[0]13.03.0
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd50.iqr[1]28.06.0
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd50.median22.04.0
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd90.defined_frac0.96947935368043090.5386538653865387
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd90.iqr[0]41.03.0
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd90.iqr[1]64.07.0
metrics.segments.whole_recording.beats.per_beat[2].apd_frames.apd90.median58.05.0
metrics.segments.whole_recording.beats.per_beat[2].apd_ms.apd50.iqr[0]13.3060388945752293.0706243602865912
metrics.segments.whole_recording.beats.per_beat[2].apd_ms.apd50.iqr[1]28.6591606960081866.1412487205731825
metrics.segments.whole_recording.beats.per_beat[2].apd_ms.apd50.median22.5179119754350054.094165813715455
metrics.segments.whole_recording.beats.per_beat[2].apd_ms.apd90.iqr[0]41.965199590583423.0706243602865912
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metrics.segments.whole_recording.beats.per_beat[2].cv.iqr_px_per_frame[0]0.2293718868345570.834968705746648
metrics.segments.whole_recording.beats.per_beat[2].cv.iqr_px_per_frame[1]3.376605844104551.817589341531979
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metrics.segments.whole_recording.beats.per_beat[2].upstroke_frame790541
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metrics.segments.whole_recording.beats.per_beat[3].activation_coverage0.11140.9999
metrics.segments.whole_recording.beats.per_beat[3].activation_spread_frames82.015.0
metrics.segments.whole_recording.beats.per_beat[3].activation_spread_ms83.9303991811668315.353121801432957
metrics.segments.whole_recording.beats.per_beat[3].apd_frames.apd50.defined_frac0.96858168761220830.65006500650065
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metrics.segments.whole_recording.beats.per_beat[3].apd_frames.apd50.iqr[1]41.07.0
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metrics.segments.whole_recording.beats.per_beat[3].apd_frames.apd90.defined_frac0.94434470377019750.5577557755775577
metrics.segments.whole_recording.beats.per_beat[3].apd_frames.apd90.iqr[0]46.753.0
metrics.segments.whole_recording.beats.per_beat[3].apd_frames.apd90.iqr[1]74.08.0
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metrics.segments.whole_recording.beats.per_beat[4].activation_spread_frames113.015.0
metrics.segments.whole_recording.beats.per_beat[4].activation_spread_ms115.660184237461615.353121801432957
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metrics.segments.whole_recording.beats.per_beat[4].apd_frames.apd50.iqr[1]35.57.0
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metrics.segments.whole_recording.beats.per_beat[4].apd_ms.apd50.iqr[0]13.3060388945752293.0706243602865912
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metrics.segments.whole_recording.beats.per_beat[4].apd_ms.apd90.iqr[0]54.2476970317297863.0706243602865912
metrics.segments.whole_recording.beats.per_beat[4].apd_ms.apd90.iqr[1]75.742067553735928.18833162743091
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sensitivity.trackers[1].segments.whole_recording.persistent_rotors01118
sensitivity.trackers[1].segments.whole_recording.singularity_count_max13374
sensitivity.trackers[1].segments.whole_recording.singularity_count_mean1.4001910219675262280.6867239732569
sensitivity.trackers[1].segments.whole_recording.singularity_frames_frac0.485195797516714431.0
sensitivity.trackers[2].persistent_rotor_tracks394
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_count_max112
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durationslength 3length 94
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].core[0]30.6740.65
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].core[1]45.4118.32
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].end1023617
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].frames_in_segment56118
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].lifetime_cycles0.376.94
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].lifetime_frames56118
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].rotation_period_frames47.0234.26
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].rotations1.1913.444
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[0].start968500
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].charge1-1
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].core[0]31.788.29
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].core[1]41.6745.56
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].end1117601
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].frames_in_segment43102
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].lifetime_cycles0.296.0
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].lifetime_frames43102
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].rotation_period_frames29.86.87
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].rotations1.44314.841
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[1].start1075500
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].core[0]33.9128.23
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].core[1]51.2194.68
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].end1210522
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].frames_in_segment5523
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].lifetime_cycles0.371.35
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].lifetime_frames5523
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].rotation_period_frames20.979.52
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].rotations2.6232.416
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_durations[2].start1156500
sensitivity.trackers[2].segments.whole_recording.persistent_rotor_frames_frac0.147086914995224441.0
sensitivity.trackers[2].segments.whole_recording.persistent_rotors394
sensitivity.trackers[2].segments.whole_recording.singularity_count_max13374
sensitivity.trackers[2].segments.whole_recording.singularity_count_mean1.4001910219675262280.6867239732569
sensitivity.trackers[2].segments.whole_recording.singularity_frames_frac0.485195797516714431.0
sensitivity.varied.baseline_frames.1.5x_cycle22526
sensitivity.varied.baseline_frames.3x_cycle45051
sensitivity.varied.baseline_frames.6x_cycle900102
sensitivity.varied.baseline_scaling.reference_cycle_frames15017
sensitivity.varied.baseline_scaling.segment_cycles_frames.whole_recording15017
sensitivity.varied.baseline_scaling.window_over_cycle_per_segment.1.5x_cycle.whole_recording1.51.53
sensitivity.varied.baseline_scaling.window_over_cycle_per_segment.run.whole_recording6.0153.0
criteria_status.rules[1].values.required_min_frames4414
criteria_status.rules[1].values.slowest_cycle_frames227
criteria_status.rules[3].descriptionTissue mask from, in order: (1) NaN in frame 0 when the recording has any NaN; (2) a mask or background-image variable shipped in the file whose shape matches the frame (binary as is, an intensity image thresholded with Otsu); (3) Otsu's threshold on the mean image, the class with more temporal signal as tissue, largest connected component kept; source, coverage and threshold recordedTissue = not NaN in frame 0 and nonzero temporal std
criteria_status.rules[3].locationworker/voltage/io.py (tissue_mask_info, otsu_threshold, largest_component)worker/voltage/io.py (tissue_mask)
criteria_status.rules[3].values.coverage0.1114[absent]
criteria_status.rules[3].values.ruleintensity variable 'bgimage' in the file, Otsu threshold 2165, class above with more temporal signal, largest connected componentnot NaN in frame 0 and nonzero std
criteria_status.rules[3].values.sideabove[absent]
criteria_status.rules[3].values.sourcefile_variable[absent]
criteria_status.rules[3].values.threshold2165.203125[absent]
criteria_status.rules[3].values.tissue_pixels11149999
criteria_status.rules[3].values.variablebgimage[absent]
criteria_status.rules[6].values.min_frames15017
criteria_status.rules[8].values.baselines.1.5x_cycle22526
criteria_status.rules[8].values.baselines.3x_cycle45051
criteria_status.rules[8].values.baselines.6x_cycle900102
criteria_status.rules[11].values.anomalies[4]baseline-window ruleRhythm detector not suitable for the expected paced period here
criteria_status.rules[11].values.anomalies[5]Rhythm detector not suitable for the expected paced period herebaseline-window rule
criteria_status.rules[13].values.unstable[0]whole_recording.singularity_frames_fracwhole_recording.persistent_rotors
criteria_status.rules[13].values.unstable[1]whole_recording.persistent_rotorswhole_recording.persistent_rotor_count_max
criteria_status.rules[13].values.unstable[2]whole_recording.persistent_rotor_count_maxwhole_recording.dominant_freq_hz
criteria_status.rules[14].values.measured_cycle15017
reportable.persistent_rotor_tracks.range_across_settings[0]0.01.0
reportable.persistent_rotor_tracks.range_across_settings[1]3.05211.0
reportable.whole_recording.dominant_freq_hz.checkedno_cited_range[absent]
reportable.whole_recording.dominant_freq_hz.range_across_settings[absent][55.989, 107.311]
reportable.whole_recording.dominant_freq_hz.settings[absent]sensitivity grid + trackers
reportable.whole_recording.dominant_freq_hz.status[absent]unstable, not reportable
reportable.whole_recording.dominant_freq_hz.value6.532[absent]
reportable.whole_recording.persistent_rotor_count_max.range_across_settings[0]0.01.0
reportable.whole_recording.persistent_rotor_count_max.range_across_settings[1]1.0124.0
reportable.whole_recording.persistent_rotors.range_across_settings[0]0.01.0
reportable.whole_recording.persistent_rotors.range_across_settings[1]3.05211.0
reportable.whole_recording.singularity_frames_frac.checked[absent]no_cited_range
reportable.whole_recording.singularity_frames_frac.range_across_settings[0.18242597898758356, 0.7459407831900668][absent]
reportable.whole_recording.singularity_frames_frac.settingssensitivity grid + trackers[absent]
reportable.whole_recording.singularity_frames_frac.statusunstable, not reportable[absent]
reportable.whole_recording.singularity_frames_frac.value[absent]1.0
rotor_count043
apd50_ms22.5179119754350054.094165813715455
apd90_ms58.3418628454452355.117707267144319
critic.checks.checks.persistent_rotor_tracks.range_across_settings[0]0.01.0
critic.checks.checks.persistent_rotor_tracks.range_across_settings[1]3.05211.0
critic.checks.checks.persistent_rotor_tracks.spread_fracNone4.66
critic.checks.checks.persistent_rotor_tracks.value_at_run_setting01118
critic.checks.checks.whole_recording.dominant_freq_hz.range_across_settings[0]6.53255.989
critic.checks.checks.whole_recording.dominant_freq_hz.range_across_settings[1]6.532107.311
critic.checks.checks.whole_recording.dominant_freq_hz.spread_frac0.00.579
critic.checks.checks.whole_recording.dominant_freq_hz.stableTrueFalse
critic.checks.checks.whole_recording.dominant_freq_hz.statusno_cited_rangeunstable_not_reportable
critic.checks.checks.whole_recording.dominant_freq_hz.value_at_run_setting6.53255.989
critic.checks.checks.whole_recording.persistent_rotor_count_max.range_across_settings[0]0.01.0
critic.checks.checks.whole_recording.persistent_rotor_count_max.range_across_settings[1]1.0124.0
critic.checks.checks.whole_recording.persistent_rotor_count_max.spread_fracNone2.86
critic.checks.checks.whole_recording.persistent_rotor_count_max.value_at_run_setting043
critic.checks.checks.whole_recording.persistent_rotors.range_across_settings[0]0.01.0
critic.checks.checks.whole_recording.persistent_rotors.range_across_settings[1]3.05211.0
critic.checks.checks.whole_recording.persistent_rotors.spread_fracNone4.66
critic.checks.checks.whole_recording.persistent_rotors.value_at_run_setting01118
critic.checks.checks.whole_recording.singularity_frames_frac.range_across_settings[0]0.182425978987583561.0
critic.checks.checks.whole_recording.singularity_frames_frac.range_across_settings[1]0.74594078319006681.0
critic.checks.checks.whole_recording.singularity_frames_frac.spread_frac1.1760.0
critic.checks.checks.whole_recording.singularity_frames_frac.stableFalseTrue
critic.checks.checks.whole_recording.singularity_frames_frac.statusunstable_not_reportableno_cited_range
critic.checks.checks.whole_recording.singularity_frames_frac.value_at_run_setting0.485195797516714431.0
critic.checks.unstable[0]whole_recording.singularity_frames_fracwhole_recording.persistent_rotors
critic.checks.unstable[1]whole_recording.persistent_rotorswhole_recording.persistent_rotor_count_max
critic.checks.unstable[2]whole_recording.persistent_rotor_count_maxwhole_recording.dominant_freq_hz
critic.stability.persistent_rotor_tracks.max3.05211.0
critic.stability.persistent_rotor_tracks.median0.01118.0
critic.stability.persistent_rotor_tracks.min0.01.0
critic.stability.persistent_rotor_tracks.spread_fracNone4.66
critic.stability.persistent_rotor_tracks.values_grid[0]05
critic.stability.persistent_rotor_tracks.values_grid[1]01161
critic.stability.persistent_rotor_tracks.values_grid[2]04500
critic.stability.persistent_rotor_tracks.values_grid[3]06
critic.stability.persistent_rotor_tracks.values_grid[4]01239
critic.stability.persistent_rotor_tracks.values_grid[5]15175
critic.stability.persistent_rotor_tracks.values_grid[6]01
critic.stability.persistent_rotor_tracks.values_grid[7]01297
critic.stability.persistent_rotor_tracks.values_grid[8]05211
critic.stability.persistent_rotor_tracks.values_grid[9]17
critic.stability.persistent_rotor_tracks.values_grid[10]01118
critic.stability.persistent_rotor_tracks.values_grid[11]03969
critic.stability.persistent_rotor_tracks.values_trackers[0]0327
critic.stability.persistent_rotor_tracks.values_trackers[1]01118
critic.stability.persistent_rotor_tracks.values_trackers[2]394
critic.stability.whole_recording.dominant_freq_hz.max6.532107.311
critic.stability.whole_recording.dominant_freq_hz.median6.53288.6485
critic.stability.whole_recording.dominant_freq_hz.min6.53255.989
critic.stability.whole_recording.dominant_freq_hz.spread_frac0.00.579
critic.stability.whole_recording.dominant_freq_hz.stableTrueFalse
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.102[absent]105.445
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.2256.532[absent]
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.26[absent]71.852
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.4506.532[absent]
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.51[absent]107.311
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.9006.532[absent]
critic.stability.whole_recording.dominant_freq_hz.values_by_baseline.9016.53255.989
critic.stability.whole_recording.persistent_rotor_count_max.max1.0124.0
critic.stability.whole_recording.persistent_rotor_count_max.median0.043.0
critic.stability.whole_recording.persistent_rotor_count_max.min0.01.0
critic.stability.whole_recording.persistent_rotor_count_max.spread_fracNone2.86
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[0]03
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[1]048
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[2]0114
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[3]02
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[4]049
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[5]1119
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[6]01
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[7]043
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[8]0124
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[9]12
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[10]043
critic.stability.whole_recording.persistent_rotor_count_max.values_grid[11]0118
critic.stability.whole_recording.persistent_rotor_count_max.values_trackers[0]010
critic.stability.whole_recording.persistent_rotor_count_max.values_trackers[1]043
critic.stability.whole_recording.persistent_rotor_count_max.values_trackers[2]112
critic.stability.whole_recording.persistent_rotor_present.stableFalseTrue
critic.stability.whole_recording.persistent_rotor_present.stable_across_filtersFalseTrue
critic.stability.whole_recording.persistent_rotor_present.stable_across_trackersFalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[0]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[1]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[2]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[3]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[4]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[6]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[7]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[8]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[10]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_grid[11]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_trackers[0]FalseTrue
critic.stability.whole_recording.persistent_rotor_present.values_trackers[1]FalseTrue
critic.stability.whole_recording.persistent_rotors.max3.05211.0
critic.stability.whole_recording.persistent_rotors.median0.01118.0
critic.stability.whole_recording.persistent_rotors.min0.01.0
critic.stability.whole_recording.persistent_rotors.spread_fracNone4.66
critic.stability.whole_recording.persistent_rotors.values_grid[0]05
critic.stability.whole_recording.persistent_rotors.values_grid[1]01161
critic.stability.whole_recording.persistent_rotors.values_grid[2]04500
critic.stability.whole_recording.persistent_rotors.values_grid[3]06
critic.stability.whole_recording.persistent_rotors.values_grid[4]01239
critic.stability.whole_recording.persistent_rotors.values_grid[5]15175
critic.stability.whole_recording.persistent_rotors.values_grid[6]01
critic.stability.whole_recording.persistent_rotors.values_grid[7]01297
critic.stability.whole_recording.persistent_rotors.values_grid[8]05211
critic.stability.whole_recording.persistent_rotors.values_grid[9]17
critic.stability.whole_recording.persistent_rotors.values_grid[10]01118
critic.stability.whole_recording.persistent_rotors.values_grid[11]03969
critic.stability.whole_recording.persistent_rotors.values_trackers[0]0327
critic.stability.whole_recording.persistent_rotors.values_trackers[1]01118
critic.stability.whole_recording.persistent_rotors.values_trackers[2]394
critic.stability.whole_recording.singularity_frames_frac.max0.74594078319006681.0
critic.stability.whole_recording.singularity_frames_frac.median0.47898758357211081.0
critic.stability.whole_recording.singularity_frames_frac.min0.182425978987583561.0
critic.stability.whole_recording.singularity_frames_frac.spread_frac1.1760.0
critic.stability.whole_recording.singularity_frames_frac.stableFalseTrue
critic.stability.whole_recording.singularity_frames_frac.values_grid[0]0.74498567335243551.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[1]0.485195797516714431.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[2]0.202483285577841451.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[3]0.74021012416427891.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[4]0.472779369627507161.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[5]0.20725883476599811.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[6]0.70582617000955111.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[7]0.44699140401146131.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[8]0.182425978987583561.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[9]0.74594078319006681.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[10]0.485195797516714431.0
critic.stability.whole_recording.singularity_frames_frac.values_grid[11]0.20343839541547281.0
critic.persistence_variants.measured_cycle.min_frames15017
critic.trackers.trackers[0].persistent_rotors_by_segment.whole_recording0327
critic.trackers.trackers[1].persistent_rotors_by_segment.whole_recording01118
critic.trackers.trackers[2].persistent_rotors_by_segment.whole_recording394
critic.required_review_topics[0]unstable:whole_recording.singularity_frames_fracunstable:whole_recording.persistent_rotors
critic.required_review_topics[1]unstable:whole_recording.persistent_rotorsunstable:whole_recording.persistent_rotor_count_max
critic.required_review_topics[2]unstable:whole_recording.persistent_rotor_count_maxunstable:whole_recording.dominant_freq_hz
critic.verdict.send_backTrueFalse
critic.verdict.metric_statuses[1]whole_recording.dominant_freq_hz:no_cited_rangewhole_recording.dominant_freq_hz:unstable_not_reportable
critic.verdict.metric_statuses[4]whole_recording.singularity_frames_frac:unstable_not_reportablewhole_recording.singularity_frames_frac:no_cited_range
pipeline.params.tracking.min_frames15017
pipeline.params.window.frame_rate_source.byproject leadproject lead
pipeline.params.window.segment_cycles_frames.whole_recording15017
pipeline.params.window.segments[0].defined_byadmission (project lead); no comparison definedadmission (project lead); no comparison defined
pipeline.baseline_rule.cycles.whole_recording.cycle_frames227
pipeline.baseline_rule.cycles.whole_recording.interval_quartiles_frames[0]12.06.0
pipeline.baseline_rule.cycles.whole_recording.interval_quartiles_frames[1]22.257.0
pipeline.baseline_rule.cycles.whole_recording.interval_quartiles_frames[2]54.07.0
pipeline.baseline_rule.cycles.whole_recording.pixels_agreeing_frac0.099640933572710950.8584858485848584
pipeline.baseline_rule.ratio40.955128.714
pipeline.baseline_rule.required_min_frames4414
pipeline.baseline_rule.slowest_cycle_frames227
pipeline.deposit_checks.measures.dv_lag1_autocorr_median-0.022236137855436912-0.49938253876247546
pipeline.deposit_checks.measures.neighbour_dv_corr_median0.40097108440635930.06412793593881308
pipeline.check.anomalies[0].effect_on_analysisThe run should use 977.0 Hz from the recording-row admission source, but the cited paper text refers to the custom/iDS system and 200x200 acquisition while this deposited file is labelled MiCAM and is 100x100. Frame-based protocol parameters are unchanged; any time conversion or rate interpretation should note the medium-confidence provenance.The fixed pipeline can run with the admitted working frame rate of 977.0 Hz, but the provenance is indirect for this MiCAM, 100x100 deposited file because the cited paper statement is for the custom iDS system and 200x200 acquisition. Time-based interpretation should therefore cite this caveat.
pipeline.check.anomalies[1].effect_on_analysisThe measured polarity is consistently non-inverted although Di-4-ANEPPS normally dims on depolarization, implying a sign flip or other transformation before deposit/export. The fixed pipeline orients from the measurement, so this does not by itself invalidate the run, but polarity-dependent interpretation should be cautious.The deposited signal appears non-inverted despite the paper naming Di-4-ANEPPS, which normally dims on depolarization. The downstream pipeline orients recordings from the measurement, so this does not by itself invalidate analysis, but polarity and any sign-dependent interpretation should be treated as pre-conditioned before deposit.
pipeline.check.anomalies[2].effect_on_analysisAll sampled values are negative integers, suggesting baseline subtraction, normalization, or other processing before deposit. The protocol can still run, but amplitudes, noise estimates, and comparisons to raw fluorescence are affected by undocumented prior processing.All sampled values are negative integers, suggesting baseline subtraction, normalization, or another file-level transformation before deposit. This means amplitude baselines and noise estimates may not reflect raw fluorescence counts, and the protocol's analysis is applied to a processed deposited array.
pipeline.check.anomalies[3].effect_on_analysisThe analysis mask is based only on nonzero temporal standard deviation and covers 9999/10000 pixels, so spatial maps may include non-tissue/background pixels. Analyses that require tissue-only pixels need a biological mask before interpretation.The analysis mask covers nearly the whole image and is based only on nonzero temporal standard deviation, not a biological heart segmentation. Spatial maps from the fixed pipeline may include non-tissue/background pixels unless a biological mask is generated for interpretation outside this run.
pipeline.check.anomalies[4].effect_on_analysisThe coded baseline rule passed: baseline_frames 901 is at least 2.0 times the slowest segment cycle reported by the rule, with ratio 40.955. This rule is provisional and is reported as informational only.The short dominant cycles reported by rhythm tools should not be used as physiology or for frame-rate inference because the expected paced period from the file label and paper is outside the rhythm tool's baseline-correction window; this is a reporting caveat and not a hold condition.
pipeline.check.anomalies[4].topicbaseline-window ruleRhythm detector not suitable for the expected paced period here
pipeline.check.anomalies[5].effect_on_analysisThe inspector cautions that the short-cycle rhythm result used in one detector should not be used to infer physiology or frame rate for this recording. The segment rhythm report for the protocol baseline gives the analysed window and dominant cycle separately; no expected outcome is inferred.The coded baseline-window rule passed: baseline_frames 901 is at least 2.0 times the slowest coded cycle, with ratio 128.714. Per policy this rule is reported only as informational and does not change proceed status here.
pipeline.check.anomalies[5].topicRhythm detector not suitable for the expected paced period herebaseline-window rule
pipeline.check.methods_textUsing provisional parameter set version 3 for protocol f65d786f-f194-4be2-88b8-10d3dd25d674, the provisional fixed criteria use baseline_frames=901, spatial_sigma=1.0, spatial_truncate=4.0, pad_frames=500, rotor_threshold=0.9, rotor_erode_px=3, link_px=4.0, max_gap=5, min_cycles=1.0, pair_dist_px=3.0, paired_frac=0.5, and render_step=4. The recording has 2047 frames of size 100x100; the frame rate used is 977.0 Hz from the recording-row admission source citing 10.1371/journal.pone.0259174, and the pixel size is not provided. The analysed frames are 500 to 1547 for the whole-recording analysis window, and the provisional segment definition is whole_recording from frame 0 to frame 2047, defined at admission with no detected or logged events.Using provisional protocol f65d786f-f194-4be2-88b8-10d3dd25d674 with provisional parameter set version 3, the fixed analysis parameters were baseline_frames 901, spatial_sigma 1.0, spatial_truncate 4.0, pad_frames 500, rotor_threshold 0.9, rotor_erode_px 3, link_px 4.0, max_gap 5, min_cycles 1.0, pair_dist_px 3.0, paired_frac 0.5, and render_step 4. The recording has 2047 frames of 100 by 100 pixels in mat format; the frame rate used was 977.0 Hz from the recording-row admission entry citing 10.1371/journal.pone.0259174, and the pixel size was not available. The analysed window used for rhythm reporting was frames 500 to 1547, and the provisional segment definition was one whole_recording segment spanning frames 0 to 2047; the parameter set, segment definitions, and criteria are provisional.
pipeline.check.rationaleThe fixed protocol is appropriate to run as-is because the recording is a mouse-labelled public reference recording under the Luther lab mouse whole-heart optical-mapping protocol, and there are no recording facts that directly conflict with specified protocol fields. The protocol does not specify a required indicator, pixel size, frame rate, or image size, so the available recording facts do not create a blocking mismatch. The main limitations are provenance and preprocessing warnings: the working frame rate is admitted as 977.0 Hz but is supported by paper text for a different named camera/resolution, and the deposited data appear transformed before deposit. These issues affect interpretation and reporting rather than requiring parameter changes under the fixed version-3 protocol.The recording may be analysed under the fixed provisional mouse whole-heart protocol as it stands. The protocol does not specify a conflicting fixed indicator, pixel size, frame rate, or image size, and the admitted 977.0 Hz frame rate is the working value on record for this file, although its MiCAM-specific provenance is only medium confidence. The main limitations are not parameter mismatches but provenance and preprocessing warnings: the deposited array appears sign/conditioning transformed, all-negative, and masked nearly as the full image, so the fixed parameters can be applied but results must be interpreted as applying to the processed deposited recording rather than raw fluorescence or a tissue-only mask.
pipeline.workers49
methods_textUsing provisional parameter set version 3 for protocol f65d786f-f194-4be2-88b8-10d3dd25d674, the provisional fixed criteria use baseline_frames=901, spatial_sigma=1.0, spatial_truncate=4.0, pad_frames=500, rotor_threshold=0.9, rotor_erode_px=3, link_px=4.0, max_gap=5, min_cycles=1.0, pair_dist_px=3.0, paired_frac=0.5, and render_step=4. The recording has 2047 frames of size 100x100; the frame rate used is 977.0 Hz from the recording-row admission source citing 10.1371/journal.pone.0259174, and the pixel size is not provided. The analysed frames are 500 to 1547 for the whole-recording analysis window, and the provisional segment definition is whole_recording from frame 0 to frame 2047, defined at admission with no detected or logged events.Using provisional protocol f65d786f-f194-4be2-88b8-10d3dd25d674 with provisional parameter set version 3, the fixed analysis parameters were baseline_frames 901, spatial_sigma 1.0, spatial_truncate 4.0, pad_frames 500, rotor_threshold 0.9, rotor_erode_px 3, link_px 4.0, max_gap 5, min_cycles 1.0, pair_dist_px 3.0, paired_frac 0.5, and render_step 4. The recording has 2047 frames of 100 by 100 pixels in mat format; the frame rate used was 977.0 Hz from the recording-row admission entry citing 10.1371/journal.pone.0259174, and the pixel size was not available. The analysed window used for rhythm reporting was frames 500 to 1547, and the provisional segment definition was one whole_recording segment spanning frames 0 to 2047; the parameter set, segment definitions, and criteria are provisional.
Statusdone
Stagedone
Heartbeat19:01:51
Log3 entries