mouse_2609_150ms_control_MiCAM, Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_MiCAM
100 × 100 px, 2,047 frames, 0.977 kHz, pixel size not stated, deposit checks: negative values
Run 6e21b7f8, 2026-10-28 18:15 UTC, protocol parameters v3, code 008c2dc (uncommitted changes), 9 workers, signature 804ddd67, run view 6e21b7f8, 2 signatures of this recording
Not reportable: range check not passed, code uncommitted changes, parameters unsigned, reviewer sign-off pending; 16 rules open
Properties
| Deposit | 10.5281/zenodo.5557829 |
| Publication | 10.1371/journal.pone.0259174 |
| Licence | cc-by-4.0 (as stated: cc-by-4.0) |
| Authors | Rybashlykov Dmitry |
| File | uploads/public/7764050e/mouse_2609_150ms_control_MiCAM.mat, mat, 30,452,226 bytes |
| sha256 | 6f7fd1ea3f1f2610645c5d235dd4bc189bcdf58c1a038c8f8e68e2233948253e |
| Frame rate | 977.0 Hz, admission 10.1371/journal.pone.0259174 |
| Pixel size | None µm |
| Polarity | upright, measured |
| Segments source | admission (project lead); no comparison defined |
| Protocol | Mouse whole heart optical mapping (Luther lab), mouse, whole heart; parameters v3 set by developer (not a domain scientist): version 3: baseline 901 frames = 2.1 x the 433-frame (2.31 Hz) post-termination cycle resolved under v2; v2's 641 frames was 1.48 cycles, on the 1.5-cycle line, and the pipeline check held one run and passed another on the same inputs. pad 500 >= half the window. Provisional (REVIEW_NEEDED item 16). |
| Run | 6e21b7f8-7cd1-499c-8e8f-ab37b36c7113 |
| Started, finished | 2026-10-28 18:15 UTC, 2026-10-28 18:18 UTC |
| Code | 008c2dc076bcebf9e7fa01a05e8b7c4b5a713b37 (uncommitted changes) |
| Workers | 9 |
| Steps | load, deposit_checks, baseline_correct, polarity, spatial_filter(phase only), hilbert_phase, phase_singularities, track_and_pair, beats_apd_cv, sensitivity, render |
| Signature | 804ddd67-f7b9-45c6-a823-bef795065584, 2026-10-28 18:17 UTC |
| Movie | runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/movie.mp4 |
| Lossless frames | runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/voltage.npy, runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/phase.npy, runs/6e21b7f8-7cd1-499c-8e8f-ab37b36c7113/frames/maps.npz; shape 1047 × 100 × 100, <f4 |
Parameters
% Mouse whole heart optical mapping (Luther lab), v3, read-only % set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16 baseline_frames = 901; % >= 2 x slowest cycle (7, images): ok pad_frames = 500; % each side, outside the window spatial_sigma = 1.0; % px, Gaussian, phase only spatial_truncate = 4.0; % kernel radius, sigma lowpass = []; % none polarity = 1; % measured, skew of dV/dt rotor_threshold = 0.9; % turns, |winding| per plaquette rotor_erode_px = 3; % px from tissue edge ignored link_px = 4.0; % tracking, max step per frame max_gap = 5; % frames a track may vanish min_cycles = 1.0; % own rotations, min_frames = 17 pair_dist_px = 3.0; % opposite-charge pairing paired_frac = 0.5; % max paired fraction of a track render_step = 4; % movie only
Segments
| Segment | Defined | Analysed | Cycle, frames | Expected Rotors | Bounded By |
|---|---|---|---|---|---|
| Whole recording | 0–2,047 | 500–1,547 | 7 (86% of pixels) | not stated | recording start → recording end |
Frames at 2,047 total, 1.024 ms per frame. Cycle from the MAP channel of the DAQ log (median interval) when one exists, else from per-pixel dominant frequency. Boundaries, expectations and the segment design are provisional (admission (project lead); no comparison defined).
Metrics
| Metric | Unit | Whole recording | Physiological Range |
|---|---|---|---|
| Rhythm | |||
| Dominant frequency | Hz | 55.989–107.311 | none cited |
| Rotors | |||
| Persistent rotors | count | 1–5,211 | none cited |
| Max concurrent persistent rotors | count | 1–124 | none cited |
| Frames with a singularity | fraction | 1.000 | none cited |
| Beats, where a global rhythm exists | |||
| Beats | count | 63 | none cited |
| Global amplitude ratio | ratio | 2.00 | gate ≥ 0.30 provisional |
| APD50 | ms | 4 | none cited |
| APD90 | ms | 5 | none cited |
| Conduction velocity | cm/s | [not computed] | none cited |
Values at the run setting. Red: range across the sensitivity grid (12 baseline × sigma settings, 3 trackers) where the spread exceeds 25 % of the median; not reportable as a value. Beat metrics are not varied over the grid. Dominant frequency over the whole analysed window: 55.989 Hz.
| Criterion | Rule | Result |
|---|---|---|
| Design expectations | none stated for this recording's segments | no criterion to test; counts are descriptive provisional |
Sensitivity analysis
| Baseline, frames | Sigma, px | Persistent Rotors | Singularity Frames, fraction | Tracks | Rule | |
|---|---|---|---|---|---|---|
| whole recording | whole recording | singularities | persistent rotors | |||
| 6× cycle 102 | light 0.5 | 5 | 1.000 | 5 | no design expectation | no design expectation |
| 6× cycle 102 | default 1.0 | 1161 | 1.000 | 1161 | no design expectation | no design expectation |
| 6× cycle 102 | heavier 2.0 | 4500 | 1.000 | 4500 | no design expectation | no design expectation |
| 3× cycle 51 | light 0.5 | 6 | 1.000 | 6 | no design expectation | no design expectation |
| 3× cycle 51 | default 1.0 | 1239 | 1.000 | 1239 | no design expectation | no design expectation |
| 3× cycle 51 | heavier 2.0 | 5175 | 1.000 | 5175 | no design expectation | no design expectation |
| 1.5× cycle 26 | light 0.5 | 1 | 1.000 | 1 | no design expectation | no design expectation |
| 1.5× cycle 26 | default 1.0 | 1297 | 1.000 | 1297 | no design expectation | no design expectation |
| 1.5× cycle 26 | heavier 2.0 | 5211 | 1.000 | 5211 | no design expectation | no design expectation |
| run 901 | light 0.5 | 7 | 1.000 | 7 | no design expectation | no design expectation |
| run 901 | default 1.0 | 1118 | 1.000 | 1118 | no design expectation | no design expectation |
| run 901 | heavier 2.0 | 3969 | 1.000 | 3969 | no design expectation | no design expectation |
Bold row: the run setting. Baselines are multiples of the slowest segment's cycle (17 frames, whole recording); the run setting is the protocol's own window. Sigma is the spatial Gaussian for phase only. Fixed: window 500–1547, polarity 1, winding threshold 0.9, edge 3 px, lifetime ≥ 17 frames. Red: a rule whose result differs from the run setting. All labels provisional.
Trackers
| Tracker | Link, px | Gap, frames | Persistent Rotors | Tracks | Persistent-Rotor Rule |
|---|---|---|---|---|---|
| whole recording | |||||
| strict | 2.0 | 1 | 327 | 327 | no design expectation |
| default, run | 4.0 | 5 | 1118 | 1118 | no design expectation |
| loose | 6.0 | 10 | 94 | 94 | no design expectation |
Tracker disagreement is a review item, not part of the pass/fail.
Stability
| Metric | Min | Median | Max | Spread / Median | Settings | Varied Over | Result |
|---|---|---|---|---|---|---|---|
| persistent rotor tracks | 1 | 1.12e+03 | 5.21e+03 | 466 % | 15 | filter grid + trackers | unstable, not reportable |
| whole recording, dominant freq (Hz) | 56 | 88.6 | 107 | 58 % | 4 | baseline windows | unstable, not reportable |
| whole recording, persistent rotors | 1 | 1.12e+03 | 5.21e+03 | 466 % | 15 | filter grid + trackers | unstable, not reportable |
| whole recording, singularity frames fraction | 1 | 1 | 1 | 0 % | 12 | filter grid | stable |
| whole recording, persistent rotor count max | 1 | 43 | 124 | 286 % | 15 | filter grid + trackers | unstable, not reportable |
A metric is reportable as a value only if its spread across all settings is at most 25 % of its median provisional; otherwise its range is reported instead.