masked_data_RG_80nM, run 82fe7343
Status done, stage done; requested by [not recorded]; worker [not recorded]; started 2026-10-27 23:46 UTC, finished 2026-10-27 23:48 UTC, 121 s; signature 3b8cc8b6
Stages
| Stage | Started | Seconds |
|---|---|---|
| [no stage log; run predates stage timing] | ||
Log
| Time | Agent | Action | Detail |
|---|---|---|---|
| 23:46:44 | runs | run started | {"checked_by": "no agent", "protocol_params_version": 1, "recording_id": "73e4ebe2-6b09-4582-a1d6-ded901b7d124", "run_id": "82fe7343-d416-4536-b39e-19b7eca30443"} |
| 23:48:45 | runs | signature written | {"criteria": "provisional", "holds": null, "run_id": "82fe7343-d416-4536-b39e-19b7eca30443", "signature_id": "3b8cc8b6-ef45-4633-9365-0217c5c54720"} |
| 13:59:29 | frames | frames backfilled | {"run_id": "82fe7343-d416-4536-b39e-19b7eca30443", "sha256": {"maps": "24aa2dfc2134c34fc0dcf070a96b7af4d65e64996b35708e5b5823496617ece8", "phase": "8299faa3c46a5bedbc0fad024fdf743b54614eac101f3f15181c0afe29cd1b13", "voltage": "79f7a050312d4d65996774bdd45837eeb483ad1547e057aecdee021b345d9f3e"}, "shape": [3400, 128, 80], "signature_id": "3b8cc8b6-ef45-4633-9365-0217c5c54720"} |
Entries from the audit log for this run; new rows and stage changes arrive by realtime while the run is open (connecting).
Pipeline check
[no pipeline check stored for this run]
Inspector
This is a preprocessed ex vivo Langendorff rat whole-heart VF optical-mapping recording under 80 nM rotigaptide, recorded at 1000 Hz according to the deposit; that agrees with the value already on record. The 128 × 80 deposited image size matches both the deposit and the linked paper, and the paper also reports 1000 frames/s. The data have documented preprocessing before deposit, including background subtraction, 3 × 3 spatial binning, 100 Hz low-pass filtering, drift removal, and normalization, so analyses should not treat them as raw camera counts. Measured polarity is consistently non-inverted even though RH237 normally dims on depolarization, so use measured polarity for orientation. No acquisition-log or detected event annotations are available for this recording.
| Measurement | Value | Method, Caveat |
|---|---|---|
| SNR, median (p10–p90) | 18.8 (13.9–25.9) | assumes white noise; on temporally smoothed deposits noise is underestimated and SNR inflated |
| Mask coverage | 50.9%, 5216 of 10240 px | depositor NaN mask (frame 0) |
| Polarity | blocks 7, inverted False, blocks agreeing 7, median dvdt skew 0.08132843673229218 | sign of median per-pixel skew of dV/dt, baseline-corrected (121 frames) |
| Drift / bleaching | baseline change -0.0108, vs beat amplitude -0.0979, slow range 0.162, trend, Spearman -0.356 | running median (381 frames) of the tissue-mean raw signal; steps from events (light, rhythm change) and depositor normalization or drift removal change or erase the baseline; bleaching cannot be separated from them here |
| Prior processing | unit range, non integer, nan mask partial, temporal smoothing, spatial smoothing | deposit checks |
| Dominant cycle by block, frames | 0–1000: 29.4, 1000–2000: 28.6, 2000–3000: 28.6, 3000–3800: 25.0 |
| Concern | Severity | Detail |
|---|---|---|
| preprocessed deposit | warn | The deposit is not raw camera data: it is documented as background-subtracted, spatially binned, low-pass filtered, drift-removed, and the publication says normalized. Analyses requiring raw fluorescence/counts or unfiltered noise statistics would be misleading. |
| polarity transformed relative to dye | warn | RH237 normally dims on depolarization, but measured polarity is non-inverted with 7/7 blocks agreeing. The depositor appears to have flipped or transformed the sign; the pipeline should orient from the measured polarity, so this is not expected to make activation analysis wrong if handled. |
| SNR depends on preprocessing | info | Median SNR is high, but documented temporal filtering and detected temporal smoothing can inflate the SNR estimate by reducing apparent frame-to-frame noise. |
| reference thresholds provisional | info | No frame-rate inference was needed because the deposit states 1 kHz. Any conclusions from automated processing flags and provisional rhythm/reference-rate tools should be treated as not yet domain-scientist reviewed. |
Inspector report 2026-10-27 20:27 UTC, model openai:gpt-5.5, provisional. Median per-pixel SNR over tissue is 18.818067017408147, with p10 = 13.893293967898506 and p90 = 25.936276941781777 over 3000 frames. The SNR tool cautions that temporal smoothing underestimates noise and inflates SNR; that caveat applies because low-pass filtering is documented and temporal smoothing was detected. The tissue mask contains 5216 pixels out of 10240 total pixels, coverage 0.509375. The mask source is a depositor NaN mask in frame 0; frame 0 has 5024 NaN pixels. No stored events were returned, no acquisition log is present, and no detected light transitions or image baseline-step events were returned. Therefore there are no event frames to interpret for this recording. If events are later added, image-only baseline steps should be treated only as fluorescence changes unless a log shows a light transition at the same frame. The tissue-mean slow baseline, measured with a 381-frame running median, changes from 0.4981755316257477 to 0.48741528391838074, a change of -0.010760247707366943, equal to -0.09788043294092134 of the p5–p95 beat amplitude. Slow baseline range is 0.1622154712677002 and Spearman trend is -0.3557674750723145. Because the deposit documents drift removal and normalization, residual bleaching cannot be separated from preprocessing effects here.
Range checks
| Metric | Segment | Value at Run Setting | Cited Range | Status |
|---|---|---|---|---|
| persistent rotor tracks | all | 303 count | none cited | unstable not reportable |
| dominant freq (Hz) | whole recording | 40.29 Hz | none cited | no cited range |
| persistent rotors | whole recording | 303 count | none cited | unstable not reportable |
| singularity frames fraction | whole recording | 1 fraction of frames | none cited | no cited range |
| persistent rotor count max | whole recording | 15 count | none cited | unstable not reportable |
Compare with run e41d035d
A: this run, code 905d5be (uncommitted changes), 9 workers. B: run e41d035d, 2026-10-27 23:43 UTC, code 905d5be (uncommitted changes), None workers. 1 stored values differ.
| File | A | B | Same | How |
|---|---|---|---|---|
| frames/voltage | 79f7a050312d | [none] | [not comparable] | recorded sha256 |
| frames/phase | 8299faa3c46a | [none] | [not comparable] | recorded sha256 |
| frames/maps | 24aa2dfc2134 | [none] | [not comparable] | recorded sha256 |
| maps/dominant_frequency.png | 8c71c54e3940 | 8c71c54e3940 | yes | sha256 of the stored file |
| movie.mp4 | 10906397 | 10906397 | yes | size in bytes only; frames not compared |
| Stored Value | A | B |
|---|---|---|
| pipeline.workers | 9 | None |