masked_data_RG_80nM, Rat heart, ventricular fibrillation
128 × 80 px, 3,800 frames, 1 kHz, 112.5 µm/px, deposit checks: unit range, non integer, nan mask partial, temporal smoothing, spatial smoothing
Run 82fe7343, 2026-10-27 23:46 UTC, protocol parameters v1, code 905d5be (uncommitted changes), 9 workers, signature 3b8cc8b6, run view 82fe7343, 9 signatures of this recording
Not reportable: range check not passed, code uncommitted changes, parameters unsigned, reviewer sign-off pending; 15 rules open
Properties
| Deposit | 10.5281/zenodo.18319102, https://zenodo.org/records/18319102 |
| Publication | 10.1093/cvr/cvaa141 |
| Licence | CC-BY-4.0 (as stated: cc-by-4.0) |
| Authors | Verstraeten, Bjorn; Lootens, Sebastiaan |
| File | public/masked_data_RG_80nM.mat, mat, 293,204,250 bytes |
| sha256 | 9e30977de373f78d1b5d236bd100e46bb79c72152d3a7516b9c03c4849391076 |
| Frame rate | 1000 Hz, deposit metadata 10.5281/zenodo.18319102 |
| Pixel size | 112.5 µm, deposit metadata 10.5281/zenodo.18319102, midpoint of the stated 100-125 um; conduction velocities carry a +/-11% range from this alone |
| Polarity | upright, measured |
| Segments source | developer (no comparison defined) |
| Protocol | Rat whole heart optical mapping (Zenodo 18319102), rat, whole heart; parameters v1 set by developer (not a domain scientist): version 1: the library defaults as of commit 363372b; provisional, see protocol_params.py |
| Run | 82fe7343-d416-4536-b39e-19b7eca30443 |
| Started, finished | 2026-10-27 23:46 UTC, 2026-10-27 23:48 UTC |
| Code | 905d5bebbb29da7ad46cd5045db14330695037c3 (uncommitted changes) |
| Workers | 9 |
| Steps | load, deposit_checks, baseline_correct, polarity, spatial_filter(phase only), hilbert_phase, phase_singularities, track_and_pair, beats_apd_cv, sensitivity, render |
| Signature | 3b8cc8b6-ef45-4633-9365-0217c5c54720, 2026-10-27 23:48 UTC |
| Movie | runs/82fe7343-d416-4536-b39e-19b7eca30443/movie.mp4 |
| Lossless frames | runs/82fe7343-d416-4536-b39e-19b7eca30443/frames/voltage.npy, runs/82fe7343-d416-4536-b39e-19b7eca30443/frames/phase.npy, runs/82fe7343-d416-4536-b39e-19b7eca30443/frames/maps.npz; shape 3400 × 128 × 80, <f4 |
Parameters
% Rat whole heart optical mapping (Zenodo 18319102), v1, read-only % set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16 baseline_frames = 121; % >= 2 x slowest cycle (26, images): ok pad_frames = 200; % each side, outside the window spatial_sigma = 1.0; % px, Gaussian, phase only spatial_truncate = 4.0; % kernel radius, sigma lowpass = []; % none polarity = 1; % measured, skew of dV/dt rotor_threshold = 0.9; % turns, |winding| per plaquette rotor_erode_px = 3; % px from tissue edge ignored link_px = 4.0; % tracking, max step per frame max_gap = 5; % frames a track may vanish min_cycles = 1.0; % own rotations, min_frames = 25 pair_dist_px = 3.0; % opposite-charge pairing paired_frac = 0.5; % max paired fraction of a track render_step = 4; % movie only
Segments
| Segment | Defined | Analysed | Cycle, frames | Expected Rotors | Bounded By |
|---|---|---|---|---|---|
| Ventricular fibrillation (whole recording) | 0–3,800 | 200–3,600 | 26 (86% of pixels) | not stated | recording start → recording end |
Frames at 3,800 total, 1.0 ms per frame. Cycle from the MAP channel of the DAQ log (median interval) when one exists, else from per-pixel dominant frequency. Boundaries, expectations and the segment design are provisional (developer (no comparison defined)).
Metrics
| Metric | Unit | Ventricular fibrillation (whole recording) | Physiological Range |
|---|---|---|---|
| Rhythm | |||
| Dominant frequency | Hz | 40.294 | none cited |
| Rotors | |||
| Persistent rotors | count | 204–464 | none cited |
| Max concurrent persistent rotors | count | 9–21 | none cited |
| Frames with a singularity | fraction | 1.000 | none cited |
| Beats, where a global rhythm exists | |||
| Beats | count | 0 | none cited |
| Global amplitude ratio | ratio | 0.28 | gate ≥ 0.30 provisional |
| APD50 | ms | no global beat | none cited |
| APD90 | ms | no global beat | none cited |
| Conduction velocity | cm/s | no global beat | none cited |
Values at the run setting. Red: range across the sensitivity grid (12 baseline × sigma settings, 3 trackers) where the spread exceeds 25 % of the median; not reportable as a value. Beat metrics are not varied over the grid. Dominant frequency over the whole analysed window: 40.294 Hz. No global upstroke found (no beat-wise activation map, CV or APD for this segment).
| Criterion | Rule | Result |
|---|---|---|
| Design expectations | none stated for this recording's segments | no criterion to test; counts are descriptive provisional |
| ROI | x | y | Value at Frame |
|---|
Figure 1. Frames 200–3,600 of 3,800, native 128 × 80 px, nearest-neighbour, no interpolation, float32 as stored. Camera: values as deposited. Voltage: baseline-corrected only (121-frame centred moving average subtracted per pixel), polarity ×1, no spatial filter. Phase: angle of the Hilbert transform of the Gaussian-filtered signal (σ = 1.0 px), twilight. Activation and dominant frequency: per-pixel maps of the current segment (magma). Singularities (open −1, filled +1): |winding| > 0.9 turns, ≥ 3 px inside the tissue edge. Rotor tracks: persistent rotors (≥ 1 own rotation, lifetime ≥ 25 frames) drawn as rings at their mean core while alive. Mask outline: tissue mask, source nan_frame0 (NaN rule; run predates the mask rule). Opening frame 694, display choice: frame with the most concurrent persistent rotors (15) in ventricular fibrillation (whole recording), midpoint of the longest such stretch. Display range, display choice: voltage ±99th percentile |V| and camera 1st–99th percentile of the current segment's tissue pixels; phase −π to π.
Figure 2. Traces of the current view at the listed ROIs, from the stored arrays. ROI A (36, 61) and ROI B (18, 110): display choice: A nearest the mean persistent-rotor core, B the farthest tissue pixel from A with amplitude at or above the tissue median. Dashed lines: segment boundaries. Red: playback cursor.