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Figures Sensitivity Report

mouse_2609_150ms_control_iDS, Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_iDS

200 × 200 px, 1,954 frames, 0.977 kHz, pixel size not stated, deposit checks: negative values

Run ee804172, 2026-10-28 20:47 UTC, protocol parameters v3, code 0d2391b, 4 workers, signature 4091a5ca, run view ee804172, 2 signatures of this recording

Not reportable: range check not passed, code clean, parameters unsigned, reviewer sign-off pending; 16 rules open

Parameters

% Mouse whole heart optical mapping (Luther lab), v3, read-only
% set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16
baseline_frames  = 901;     % no baseline rule recorded for this run
pad_frames       = 500;     % each side, outside the window
spatial_sigma    = 1.0;     % px, Gaussian, phase only
spatial_truncate = 4.0;     % kernel radius, sigma
lowpass          = [];      % none
polarity         = 1;       % measured, skew of dV/dt
rotor_threshold  = 0.9;     % turns, |winding| per plaquette
rotor_erode_px   = 3;       % px from tissue edge ignored
link_px          = 4.0;     % tracking, max step per frame
max_gap          = 5;       % frames a track may vanish
min_cycles       = 1.0;     % own rotations, min_frames = 136
pair_dist_px     = 3.0;     % opposite-charge pairing
paired_frac      = 0.5;     % max paired fraction of a track
render_step      = 4;       % movie only

Segments

SegmentDefinedAnalysedCycle, framesExpected RotorsBounded By
Whole recording0–1,954500–1,454[not measured]not statedrecording start → recording end

Frames at 1,954 total, 1.024 ms per frame. Cycle from the MAP channel of the DAQ log (median interval) when one exists, else from per-pixel dominant frequency. Boundaries, expectations and the segment design are provisional (admission (project lead); no comparison defined).

Metrics

MetricUnitWhole recordingPhysiological Range
Rhythm
Dominant frequencyHz 7.169 none cited
Rotors
Persistent rotorscount 9–609 none cited
Max concurrent persistent rotorscount 1–75 none cited
Frames with a singularityfraction 1.000 none cited
Beats, where a global rhythm exists
Beatscount 0 none cited
Global amplitude ratioratio 0.28 gate ≥ 0.30 provisional
APD50ms no global beat none cited
APD90ms no global beat none cited
Conduction velocitycm/s no global beat none cited

Values at the run setting. Red: range across the sensitivity grid (12 baseline × sigma settings, 3 trackers) where the spread exceeds 25 % of the median; not reportable as a value. Beat metrics are not varied over the grid. Dominant frequency over the whole analysed window: 7.169 Hz. No global upstroke found (no beat-wise activation map, CV or APD for this segment).

CriterionRuleResult
Design expectationsnone stated for this recording's segmentsno criterion to test; counts are descriptive provisional
display only
cursorx –, y – value–
ROIxyValue at Frame

Figure 1. Frames 500–1,454 of 1,954, native 200 × 200 px, nearest-neighbour, no interpolation, float32 as stored. Camera: values as deposited. Voltage: baseline-corrected only (901-frame centred moving average subtracted per pixel), polarity ×1, no spatial filter. Phase: angle of the Hilbert transform of the Gaussian-filtered signal (σ = 1.0 px), twilight. Activation and dominant frequency: per-pixel maps of the current segment (magma). Singularities (open −1, filled +1): |winding| > 0.9 turns, ≥ 3 px inside the tissue edge. Rotor tracks: persistent rotors (≥ 1 own rotation, lifetime ≥ 136 frames) drawn as rings at their mean core while alive. Mask outline: tissue mask, source file_variable. Opening frame 623, display choice: frame with the most concurrent persistent rotors (26) in whole recording, midpoint of the longest such stretch. Display range, display choice: voltage ±99th percentile |V| and camera 1st–99th percentile of the current segment's tissue pixels; phase −π to π.

Figure 2. Traces of the current view at the listed ROIs, from the stored arrays. ROI A (59, 79) and ROI B (41, 174): display choice: A nearest the mean persistent-rotor core, B the farthest tissue pixel from A with amplitude at or above the tissue median. Dashed lines: segment boundaries. Red: playback cursor.

Frame–
Time–
Cursor–
Segment–
Rundone
Review items25 open
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