mouse_2609_150ms_control_iDS, Open-Source Low-Cost Cardiac Optical Mapping System: mouse_2609_150ms_control_iDS
200 × 200 px, 1,954 frames, 0.977 kHz, pixel size not stated, deposit checks: negative values
Run ee804172, 2026-10-28 20:47 UTC, protocol parameters v3, code 0d2391b, 4 workers, signature 4091a5ca, run view ee804172, 2 signatures of this recording
Not reportable: range check not passed, code clean, parameters unsigned, reviewer sign-off pending; 16 rules open
Properties
| Deposit | 10.5281/zenodo.5557829 |
| Publication | 10.1371/journal.pone.0259174 |
| Licence | cc-by-4.0 (as stated: cc-by-4.0) |
| Authors | Rybashlykov Dmitry |
| File | uploads/public/7764050e/mouse_2609_150ms_control_iDS.mat, mat, 114,024,466 bytes |
| sha256 | f9f4309fb395aa43ccae96f6e85724a7a739a98c295ff45598a6417b01605709 |
| Frame rate | 977.0 Hz, admission 10.1371/journal.pone.0259174 |
| Pixel size | None µm |
| Polarity | upright, measured |
| Segments source | admission (project lead); no comparison defined |
| Protocol | Mouse whole heart optical mapping (Luther lab), mouse, whole heart; parameters v3 set by developer (not a domain scientist): version 3: baseline 901 frames = 2.1 x the 433-frame (2.31 Hz) post-termination cycle resolved under v2; v2's 641 frames was 1.48 cycles, on the 1.5-cycle line, and the pipeline check held one run and passed another on the same inputs. pad 500 >= half the window. Provisional (REVIEW_NEEDED item 16). |
| Run | ee804172-2876-47fb-94df-d522ea5c6e03 |
| Started, finished | 2026-10-28 20:47 UTC, 2026-10-28 20:51 UTC |
| Code | 0d2391b04113d316716bce2e2f9ee6ec6728d5d3 |
| Workers | 4 |
| Steps | load, deposit_checks, baseline_correct, polarity, spatial_filter(phase only), hilbert_phase, phase_singularities, track_and_pair, beats_apd_cv, sensitivity, render |
| Signature | 4091a5ca-4825-4b42-bced-67d6ecc7a015, 2026-10-28 20:50 UTC |
| Movie | runs/ee804172-2876-47fb-94df-d522ea5c6e03/movie.mp4 |
| Lossless frames | runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/voltage.npy, runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/phase.npy, runs/ee804172-2876-47fb-94df-d522ea5c6e03/frames/maps.npz; shape 954 × 200 × 200, <f4 |
Parameters
% Mouse whole heart optical mapping (Luther lab), v3, read-only % set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16 baseline_frames = 901; % no baseline rule recorded for this run pad_frames = 500; % each side, outside the window spatial_sigma = 1.0; % px, Gaussian, phase only spatial_truncate = 4.0; % kernel radius, sigma lowpass = []; % none polarity = 1; % measured, skew of dV/dt rotor_threshold = 0.9; % turns, |winding| per plaquette rotor_erode_px = 3; % px from tissue edge ignored link_px = 4.0; % tracking, max step per frame max_gap = 5; % frames a track may vanish min_cycles = 1.0; % own rotations, min_frames = 136 pair_dist_px = 3.0; % opposite-charge pairing paired_frac = 0.5; % max paired fraction of a track render_step = 4; % movie only
Segments
| Segment | Defined | Analysed | Cycle, frames | Expected Rotors | Bounded By |
|---|---|---|---|---|---|
| Whole recording | 0–1,954 | 500–1,454 | [not measured] | not stated | recording start → recording end |
Frames at 1,954 total, 1.024 ms per frame. Cycle from the MAP channel of the DAQ log (median interval) when one exists, else from per-pixel dominant frequency. Boundaries, expectations and the segment design are provisional (admission (project lead); no comparison defined).
Metrics
| Metric | Unit | Whole recording | Physiological Range |
|---|---|---|---|
| Rhythm | |||
| Dominant frequency | Hz | 7.169 | none cited |
| Rotors | |||
| Persistent rotors | count | 9–609 | none cited |
| Max concurrent persistent rotors | count | 1–75 | none cited |
| Frames with a singularity | fraction | 1.000 | none cited |
| Beats, where a global rhythm exists | |||
| Beats | count | 0 | none cited |
| Global amplitude ratio | ratio | 0.28 | gate ≥ 0.30 provisional |
| APD50 | ms | no global beat | none cited |
| APD90 | ms | no global beat | none cited |
| Conduction velocity | cm/s | no global beat | none cited |
Values at the run setting. Red: range across the sensitivity grid (12 baseline × sigma settings, 3 trackers) where the spread exceeds 25 % of the median; not reportable as a value. Beat metrics are not varied over the grid. Dominant frequency over the whole analysed window: 7.169 Hz. No global upstroke found (no beat-wise activation map, CV or APD for this segment).
| Criterion | Rule | Result |
|---|---|---|
| Design expectations | none stated for this recording's segments | no criterion to test; counts are descriptive provisional |
Sensitivity analysis
| Baseline, frames | Sigma, px | Persistent Rotors | Singularity Frames, fraction | Tracks | Rule | |
|---|---|---|---|---|---|---|
| whole recording | whole recording | singularities | persistent rotors | |||
| 6× cycle 816 | light 0.5 | 12 | 1.000 | 12 | no design expectation | no design expectation |
| 6× cycle 816 | default 1.0 | 274 | 1.000 | 274 | no design expectation | no design expectation |
| 6× cycle 816 | heavier 2.0 | 329 | 0.949 | 329 | no design expectation | no design expectation |
| 3× cycle 408 | light 0.5 | 11 | 1.000 | 11 | no design expectation | no design expectation |
| 3× cycle 408 | default 1.0 | 351 | 1.000 | 351 | no design expectation | no design expectation |
| 3× cycle 408 | heavier 2.0 | 609 | 0.986 | 609 | no design expectation | no design expectation |
| 1.5× cycle 204 | light 0.5 | 16 | 1.000 | 16 | no design expectation | no design expectation |
| 1.5× cycle 204 | default 1.0 | 229 | 1.000 | 229 | no design expectation | no design expectation |
| 1.5× cycle 204 | heavier 2.0 | 305 | 0.971 | 305 | no design expectation | no design expectation |
| run 901 | light 0.5 | 9 | 1.000 | 9 | no design expectation | no design expectation |
| run 901 | default 1.0 | 308 | 1.000 | 308 | no design expectation | no design expectation |
| run 901 | heavier 2.0 | 370 | 0.944 | 370 | no design expectation | no design expectation |
Bold row: the run setting. Baselines are multiples of the slowest segment's cycle (136 frames, whole recording); the run setting is the protocol's own window. Sigma is the spatial Gaussian for phase only. Fixed: window 500–1454, polarity 1, winding threshold 0.9, edge 3 px, lifetime ≥ 136 frames. Red: a rule whose result differs from the run setting. All labels provisional.
Trackers
| Tracker | Link, px | Gap, frames | Persistent Rotors | Tracks | Persistent-Rotor Rule |
|---|---|---|---|---|---|
| whole recording | |||||
| strict | 2.0 | 1 | 80 | 80 | no design expectation |
| default, run | 4.0 | 5 | 308 | 308 | no design expectation |
| loose | 6.0 | 10 | 64 | 64 | no design expectation |
Tracker disagreement is a review item, not part of the pass/fail.
Stability
| Metric | Min | Median | Max | Spread / Median | Settings | Varied Over | Result |
|---|---|---|---|---|---|---|---|
| persistent rotor tracks | 9 | 274 | 609 | 219 % | 15 | filter grid + trackers | unstable, not reportable |
| whole recording, dominant freq (Hz) | 6.14 | 6.66 | 7.17 | 15 % | 4 | baseline windows | stable |
| whole recording, persistent rotors | 9 | 274 | 609 | 219 % | 15 | filter grid + trackers | unstable, not reportable |
| whole recording, singularity frames fraction | 0.944 | 1 | 1 | 6 % | 12 | filter grid | stable |
| whole recording, persistent rotor count max | 1 | 21 | 75 | 352 % | 15 | filter grid + trackers | unstable, not reportable |
A metric is reportable as a value only if its spread across all settings is at most 25 % of its median provisional; otherwise its range is reported instead.