Parameters

% Mouse whole heart optical mapping (Luther lab), v3, read-only
% set by developer (not a domain scientist); provisional, REVIEW_NEEDED 16
baseline_frames  = 901;     % >= 2 x slowest cycle (415, MAP): ok
pad_frames       = 500;     % each side, outside the window
spatial_sigma    = 1.0;     % px, Gaussian, phase only
spatial_truncate = 4.0;     % kernel radius, sigma
lowpass          = [];      % none
polarity         = -1;      % measured, skew of dV/dt
rotor_threshold  = 0.9;     % turns, |winding| per plaquette
rotor_erode_px   = 3;       % px from tissue edge ignored
link_px          = 4.0;     % tracking, max step per frame
max_gap          = 5;       % frames a track may vanish
min_cycles       = 1.0;     % own rotations, min_frames = 52
pair_dist_px     = 3.0;     % opposite-charge pairing
paired_frac      = 0.5;     % max paired fraction of a track
render_step      = 4;       % movie only

Segments

Frames at 9,267 total, 1.0 ms per frame. Cycle from the MAP channel of the DAQ log (median interval) when one exists, else from per-pixel dominant frequency. Boundaries, expectations and the segment design are provisional (project lead (not a domain scientist), following the paper's design, 10.1371/journal.pcbi.1011660).

Metrics

Values at the run setting. Red: range across the sensitivity grid (12 baseline × sigma settings, 3 trackers) where the spread exceeds 25 % of the median; not reportable as a value. Beat metrics are not varied over the grid. Dominant frequency over the whole analysed window: 2.419 Hz.